Sequences from confirmation amplicon


Confirmation sequences for line 035E08 and hit At5g37710 are shown.
Line ID 035E08
Locus At5g37710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:14979939
Primers used for PCR 18mm - 8474, show primer details
   
Sequence Name 62-K114263-0022-035-E08C-18mm
GenBank Accession LN510814 [GenBank]
Sequencing primer gene specific primer, 18mm: 5'-AAACCCTAAAATCTCACCTTCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K114263-0022-035-E08C-18mm
ACGAAAAAACCCAAGGAAGATCTCCACCGTACATTATCTACATAGATCACGACCACCGTG
AGATTGTTTTAGCGATTCGTGGCTTGAACTTAGCTAAAGAGAGTGATTACAAGATTCTGT
TAGATAACAAATTAGGTCAGAAAATGCTTGGTGGTGGTTATGTGCATCGTGGGTTGTTGA
AATCTGCGGCTTGGGTTTTGAATCAAGAATCTGAGACTCTTTGGAGAGTTTGGGAAGAGA
ATGGTAGAGAGTATGATTTGGTTTTTGCTGGTCATTCTTTGGGTTCTGGTGTTGCGGCGT
TGATGGCGGTTTTGGTTGTTAATACTCCGGCGATGATTGGTGATATTCCGAGGAATAAGG
TTCGATGTTTTGCTTTGGCTCCGGCGAGATGTATGTCGCTTAATCTCGCCGTTAAGTATG
CTGACGTCATCTCCTCCGTCATTTTGCAGGTAAAAAAATTTAACCATGTTTTAATGATCT
CAATGTTAGTTAAAAGATTTTTACAAAAATTGATTTTTTAACAAAGAATAATATATTAGA
AATCTACNTGGATCAGCATTGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAA
   
Sequence Name 19-K114262-0022-035-E08C-8409
GenBank Accession LN494856 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K114262-0022-035-E08C-8409
TTCTTTGTTAAAAATCAATTTTTGTAAAATCTTTTAACTAACATTGAGATCATTAAAACA
TGGTTAAATTTTTTTACCTGCAAAATGACGGAGGAGATGACGTCAGCATACTTAACGGCG
AGATTAAGCGACATACATCTCGCCGGAGCCAAAGCAAAACATCGAACCTTATTCCTCGGA
ATATCACCAATCATCGCCGGAGTATTAACAACCAAAACCGCCATCAACGCCGCAACACCA
GAACCCAAAGAATGACCAGCAAAAACCAAATCATACTCTCTACCATTCTCTTCCCAAACT
CTCCAAAGAGTCTCAGATTCTTGATTCAAAACCCAAGCCGCAGATTTCAACAACCCACGA
TGCACATAACCACCACCAAGCATTTTCTGACCTAATTTGTTATCTAACAGAATCTTGTAA
TCACTCTCTTTAGCTAAGTTCAAGCCACGAATCGCTAAAACAATCTCACGGTGGTCGTGA
TCTATGTAGATAATGTACGGTGGAGATCTTCCTTGGGTTTTTTCGTGTGTCACACGTTTG
ATGACCCATTCAGGGTTTAGATCGAAAGTTCCTAACGAAGGTGAGATTTTAGGGTT

Last Updated on 06.12.2018 16:11