Sequences from confirmation amplicon


Confirmation sequences for line 035G08 and hit At1g09575 are shown.
Line ID 035G08
Locus At1g09575 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3102073
Primers used for PCR 06uv - 8474, show primer details
   
Sequence Name 19-K117583-0022-035-G08C-06uv
GenBank Accession LN494940 [GenBank]
Sequencing primer gene specific primer, 06uv: 5'-CTAATGAGGCTTGTGAATGTTGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K117583-0022-035-G08C-06uv
GTCTCTTACTCACACTTTTAGAAGCTTCTCAAGGAATGGGTATCGCTAGATCTCCCGATG
AAGCTCATGTCTTTGCTCGTGTTCTTGACGATGCTGGCGTTGTCTTGATCTTTCGTGATA
AAGTCTATCTTCACCCAGACAAGGTCTTAATTAGAACACAAAAACAGAACTTTACTCTGT
TTTGTCTCACTTTACTCTGTTTTGTCTCGATTTCTCTAAAAATCTGGACTTTTTTGTTTT
GTTCAGGTGGTGGATCTGATTAGAAGAGCGATGCCTTTGGACCAGAATCCAGAGGAAGAT
CAAATCAAGGAAGAATTCAACAAATTGAGAATCATGAAAGAAGAGATTGATGTTTTGGCT
CATAGACAAGTGAGGAAGATTCTTTGGTGTGGTTTAGCTACTTCGATGGTTCAAATTGGA
CTCTTTTTTAGATTAACTTTCTGGGAATTCTCATGGGATGTGATGGAACCAATCACTTTC
TTCGCTACAGCGACTGGAATCATTGTGGGTTATGCTTATTTCAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATTC
   
Sequence Name 03-K117582-0022-035-G08C-8409
GenBank Accession LN487662 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117582-0022-035-G08C-8409
AATTGAATATATCCTGAAATAAGCATAACCCACAATGATTCCAGTCGCTGTAGCGAAGAA
AGTGATTGGTTCCATCACATCCCATGAGAATTCCCAGAAAGTTAATCTAAAAAAGAGTCC
AATTTGAACCATCGAAGTAGCTAAACCACACCAAAGAATCTTCCTCACTTGTCTATGAGC
CAAAACATCAATCTCTTCTTTCATGATTCTCAATTTGTTGAATTCTTCCTTGATTTGATC
TTCCTCTGGATTCTGGTCCAAAGGCATCGCTCTTCTAATCAGATCCACCACCTGAACAAA
ACAAAAAAGTCCAGATTTTTAGAGAAATCGAGACAAAACAGAGTAAAGTGAGACAAAACA
GAGTAAAGTTCTGTTTTTGTGTTCTAATTAAGACCTTGTCTGGGTGAAGATAGACTTTAT
CACGAAAGATCAAGACAACGCCAGCATCGTCAAGAACACGAGCAAAGACATGAGCTTCAT
CGGGAGATCTAGCGATACCCATTCCTTGAGAAGCTTCTAAAAGTGTAGAGTAAGAGACAA
CATCTCTATCAGCCACACCAACAAGCTTCTTCTTCATGTCTTCAACATTC

Last Updated on 06.12.2018 16:11