Sequences from confirmation amplicon


Confirmation sequences for line 035H08 and hit At5g64000 are shown.
Line ID 035H08
Locus At5g64000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25617277
Primers used for PCR 20mm - 8474, show primer details
   
Sequence Name 46-K114263-0022-035-H08C-20mm
GenBank Accession LN505935 [GenBank]
Sequencing primer gene specific primer, 20mm: 5'-GGAGCTTATATGAATCGCTGAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K114263-0022-035-H08C-20mm
CTTTTGCAATCCCAAGTTTGGAAAAATCCGATAGAAGTCCGGTTACTGCAGCTGATTACG
GTTTGTACCTCGTCGTTTGCATACCCGCGAATTCAACTTCAAGTTTCTGTTTTTGCTAAG
TTCTTGTTTTCTCTATAAAATCAATCAAGAAAATGTCTTTTACCACTCCCATTAACGGAT
GGTTTCTTCAGGCTCACAAGCTGTGGTTAGTCTTGTATTGGAGAGGGAGCTCCAACCTGA
TAAACTGTCATTAGTAGCTGAAGAGGTGAAATAACACTTGAAAACCTATATNAATCAGAG
GTTTTATAGCGTAAATGTTACTCGGGTTTTATAACGGGGAAATCTTACAGGAAACTGGAG
ATCTGCGGAAGAATGGAAGTGAGGCGTTTCTAGAAGATATTGCAAAGCTTGTGAAAGATA
CTCTAGCCTCTGAGGAATCGTACACCAGCTCTCCTCTATCCACAGATGATGTGTTAAATG
CTATATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCANAAATGTAT
   
Sequence Name 03-K114262-0022-035-H08C-8409
GenBank Accession LN487580 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K114262-0022-035-H08C-8409
TGGTGTACGATTCCTCAGAGGCTAGAGTATCTTTCACAAGCTTTGCAATATCTTCTAGAA
ACGCCTCACTTCCATTCTTCCGCAGATCTCCAGTTTCCTGTAAGATTTCCCCGTTATAAA
ACCCGAGTAACATTTACGCTATAAAACCTCTGATTAATATAGGTTTTCAAGTGTTATTTC
ACCTCTTCAGCTACTAATGACAGTTTATCAGGTTGGAGCTCCCTCTCCAATACAAGACTA
ACCACAGCTTGTGAGCCTGAAGAAACCATCCGTTAATGGGAGTGGTAAAAGACATTTTCT
TGATTGATTTTATAGAGAAAACAAGAACTTAGCAAAAACAGAAACTTGAAGTTGAATTCG
CGGGTATGCAAACGACGAGGTACAAACCGTAATCAGCTGCAGTAACCGGACTTCTATCGG
ATTTTTTCCAAACTTGGGATTGCAAAAGAGTCTTCTGCACTTCCTGATTCATTACATAAT
AAGGTTTGTTTTAGTTTCAGCGATTCA

Last Updated on 06.12.2018 16:11