Sequences from confirmation amplicon


Confirmation sequences for line 036A01 and hit At5g14740 are shown.
Line ID 036A01
Locus At5g14740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4761800
Primers used for PCR FI09 - 8474, show primer details
   
Sequence Name 08-K028094-022-036-A01C-8409
GenBank Accession LN489589 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K028094-022-036-A01C-8409
ATTTCATTGAATAATCCTGTGCACATGCACTGTGCCCTATCACCACAATGTTTTCCACCT
ACATTCATAAATTTTCAATAAACATCAACATATGTATTGATGGATAATATACTCACATCA
CATAAATCACAACACACTAAATACAGAACGGCCCAATAAGGCCCATATACTCATCGCCTT
AACCGGAGTAATAAGTAACATTGATACATATAAACTAACATGCATCCTATGAGTCAAGCA
AGTTTTAGGAAAAAACACAAAACAATCTAAAGAGAGAAGAATGTATATATTGATTATTGA
GGGAGAGATTTTGACCTTAAGGTGCAAGACAGCGTATTCAATGGCGGCTCCAACTCCTGC
ATATTTGACCTGTAATTATTTTTATAAAGGAAAAAAAAAACAGAAAAGCCAT
   
Sequence Name 08-K028094-022-036-A01C-FI09
GenBank Accession LN489590 [GenBank]
Sequencing primer gene specific primer, FI09: 5'-TGAAGTCATAACATGGCTTTTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K028094-022-036-A01C-FI19
ATTACGGTCAATATGCAGGAGTTGGAGCCGCCATTGAATACCCTGTCTTGCACCTTAAGG
TCAAAATCTCTCCCTCAATAATCAATATATACATTCTTCCCCCTTTAAATTGTTTTGGGT
TTTTTCCTAAAACTTGCTTGACTCAAAGGATGCAGGTTAGTTTATATGTATCAATGTTAC
TTATTACTCCGGTTAAGGCGATAAGTATATGGGCCTTATTGGGCCGTTCTGTATTTAGTG
TGTTGTGATTTATGTGATGTGAGTATATTATCCATCAATACATATGTTGATGTTTATTGA
AAATTTATGAATGTAGGTGGAAAACATTGTGGTGATAGGGCACAGTGCATGTGCACAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCACCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
AATGTCCGCACCGTTATTATAAAATGAAAGTACTTTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCA
ACGTGTCTACATTCC
Primers used for PCR AB75 - 8474, show primer details
   
Sequence Name 31-K011908-0022-036-A01C-8409
GenBank Accession LN499935 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K011908-0022-036-A01C-8409
TTTCCCGCCTTGGGGTTAAACTATCAGTGGTTTAAAAAAATTGGTATTTACTCTTTCTTT
TTCTCCATATTGGCCATCATACTCATTGCTGATCCATGTAGATTTCCCGGACATGAAGCC
ATTTACACATGTCCTCTTGACGGAAACAACTCTACGTAATAAATTTTCTATGTTTGACAT
TTATAAACTAGTAAATTATAACAAAGTTGTTTCACAAGATTTACTTTTTAGGAGAAATGG
TAACAAAGTGTTTTTGTGGGTAGTTATAATGATATGTTTACTTTAATTGCAGTGACTTCA
TAGAGGATTGGGTCAAAATCTGTTTACCAGCAAAGTCAAAAGTTTTGGCAGAAAGTGAAA
GTTCAGCATTTGAAGACCAATGTGGCCGATGCGAAAGGGTACGTAATACTCCATTTATTT
TTTCAGTTAACACTTTATTTATTCATTAGAATCATAGATAGTCATCAACCTTAACAAAAA
TATAAATATTGGATAATTAAATTATCTTTATCGGAAATGTTTTCTAATATGAATGTATGA
ATATTGCAGGAGGCAGTGAATGTGTCACTAGCAAACCTATTGACATATCCCATTTGTGAG
AGAAGGAGTTGTGAAAGGAACACTTGCTTTGAAGGGAGGCTACTATGACTTTGTTAATGG
CTCCTTTGAGCTTTGGGAGCTCCAGT

Last Updated on 06.12.2018 16:11