Sequences from confirmation amplicon


Confirmation sequences for line 036B02 and hit At2g37360 are shown.
Line ID 036B02
Locus At2g37360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15674703
Primers used for PCR MD11 - 8474, show primer details
   
Sequence Name 39-K024287-022-036-B02C-MD11
GenBank Accession LN503219 [GenBank]
Sequencing primer gene specific primer, MD11: 5'-GAAGAATCCTAAACGTTCTTGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K024287-022-036-B02C-MD11
TTGGGGAATCGTCTAGGTTAGTGAACATTGTAGCTAGGATGATTCCGGTGACCATTACAG
CTCCTAGTCTCATTCCGAGGAGTTCTGGTTGTCTTCTTGAGTTTAATATAGCACGTTTGC
CTATTACTATCATTTCAATCCAGAAAGGATTGGCGAATGTTTGGAATGATGGTGTTAGGT
TTGATGAGTTGTTGTTCGTTGCTCCAGAGACGAGTTTCCCTCTTGAGATACTCGCGGTTA
TCGCTTCTTTGAGAGAAGATACGTTGGTGTTTCTCTTGTTATTGTTGTTGTAGCTTGGTG
CTTGCTTTGCTCGCCATTGCTTGTGGAACTCGACAAGTGGCTTGGTTCCTTCTGTAGAAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
TGTCTACATT
   
Sequence Name 40-K024287-022-036-B02C-8409
GenBank Accession LN503574 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K024287-022-036-B02C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATTCTACAGAAGGAACCAAG
CCACTTGTCGAGTTCCACAAGCAATGGCGAGCAAAGCAAGCACCAAGCTACAACAACAAT
AACAAGAGAAACACCAACGTATCTTCTCTCAAAGAAGCGATAACCGCGAGTATCTCAAGA
GGGAAACTCGTCTCTGGAGCAACGAACAACAACTCATCAAACCTAACACCATCATTCCAA
ACATTCGCCAATCCTTTCTGGATTGAAATGATAGTAATAGGCAAACGTGCTATATTAAAC
TCAAGAAGACAACCAGAACTCCTCGGAATGAGACTAGGAGCTGTAATGGTCACCGGAATC
ATCCTAGCTACAATGTTCACTAACCTAGACAATTCCCCAAAAGGAGCACAAGAACGTTT
Primers used for PCR FL70 - 8474, show primer details
   
Sequence Name 63-K029745-022-036-B02C-8409
GenBank Accession LN510902 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K029745-022-036-B02C-8409
CATTTACAATTGCTGATCTCATCCGATCGAGAGCGAACTCTGTTTTGTTCTCGTTTTCAG
GAATTGGGTGCTTGAATTCTGAGAAGAACTGAGGGAGATGTGTTGGTGAGCCGCTGTAGA
CTGTGTTTCCCTTAGAAAGGAAGATTAACTGATCTAGAAGACCCATGATTCTGTAACTAG
GCTGATGGATTGACATGATAACTATGCTGCCACTTTGTGCGATTCTCTGAAGGACTTTGA
TAACCATGTAGGCGCTTGTAGAGTCGAGCCCTGAGGTTGGCTCATCTAGAAGAAGGATG
   
Sequence Name 64-K029745-022-036-B02C-FL70
GenBank Accession LN511115 [GenBank]
Sequencing primer gene specific primer, FL70: 5'-ATCATCCTCTTTCTAGATGAGCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K029745-022-036-B02C-FL70
TCAGCGCCTACATGGTTATCAAAGTCCTTCAGAGAATCGCACAAAGTGGCAGCATAGTTA
TCATGTCAATCCATCAGCCTAGTTACAGAATCATGGGTCTTCTAGATCAGTTAATCTTCC
TTTCTAAGGGAAACACAGTCTACAGCGGCTCACCAACACATCTCCCTCAGTTCTTCTCAG
AATTCAAGCACCCAATTCCTGAAAACGAGAACAAAACAGAGTTCGCTCTCGATCGGATGA
GATCAGCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATTC

Last Updated on 06.12.2018 16:11