Sequences from confirmation amplicon


Confirmation sequences for line 036D11 and hit At3g12560 are shown.
Line ID 036D11
Locus At3g12560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3984581
Primers used for PCR DN26 - 8474, show primer details
   
Sequence Name 02-K101828-0022-036-D11C-8409
GenBank Accession LN486974 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K101828-0022-036-D11C-8409
TTACATTGAAATATCCTGATCTTCAATGCATTTGAAGAAATTAAGCAAGAGAAAGTAGAA
AATTGCAAGACTATTAAATCTGAGTCTTCTGACCAAGGAAACTCTGTGTCAAAGCCTACT
TATGATATCTCTACTGAGAAGTGTGTGGTGAACAGTTGTTTTTCATTTCCGGATAGTGAC
GGCGTTTTGGAGCGGACTCCGATGTCTGATTACAAGAAGATTCATGGTTTGATGGATGTA
GGGTGTGAAAACAAGAATGTAAATAATGGGTTCGAGCAAGGAGAAGCAACCGATCGCGTG
GGTGATGGAGGCTTAGTCACTGATACTTGCAACTTAGAGGATGCAACTGCGTTAGGTCTG
CAGTTTCCGAAATCAGTCTGTGTGGGTGGTGATTTAAAATCACCATCCACCTTGGATATG
ACCCCTAATGGTTCCTATGCTAGACATGGGAACCATACTAACCTAGGTAGAAAAGATGAT
GATGAAAAATTCTATAGTTACCATAAACTTAGCAATAAATTTAAGTCGTATAGGTCTCCA
ACAATTCGAAGAATAAGAAAGTCCATGTC
   
Sequence Name 46-K101831-0022-036-D11C-DN26
GenBank Accession LN505819 [GenBank]
Sequencing primer gene specific primer, DN26: 5'-AGTATTTGGACGACATGGACTTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K101831-0022-036-D11C-DN26
GACTTAATTTATTGCTAAGTTTATGGTAACTATAGAATTTTTCATCATCATCTTTTCTAC
CTAGGTTAGTATGGTTCCCATGTCTAGCATAGGAACCATTAGGGGTCATATCCAAGGTGG
ATGGTGATTTTAAATCACCACCCACACAGACTGATTTCGGAAACTGCAGACCTAACGCAG
TTGCATCCTCTAAGTTGCAAGTATCAGTGACTAAGCCTCCATCACCCACGCGATCGGTTG
CTTCTCCTTGCTCGAACCCATTATTTACATTCTTGTTTTCACACCCTACATCCATCAAAC
CATGAATCTTCTTGTAATCAGACATCGGAGTCCGCTCCAAAACGCCGTCACTATCCGGAA
ATGAAAAACAACTGTTCACCACACACTTCTCAGTAGAGATATCATAAGTAGGCTTTGACA
CAGAGTTTCCTTGGTCAGAAGACTCAGATTTAATAGTCTTGCAATTTTCTACTTTCTCTT
GCTTAATTTCTTCAAATGCATTGAAGATCAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT

Last Updated on 06.12.2018 16:11