Sequences from confirmation amplicon


Confirmation sequences for line 036E11 and hit At1g35515 are shown.
Line ID 036E11
Locus At1g35515 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:13079693
Primers used for PCR DR03 - 8474, show primer details
   
Sequence Name 15-K018966-022-036-E11C-DR03
GenBank Accession LN492763 [GenBank]
Sequencing primer gene specific primer, DR03: 5'-GCACACAACTCCAAAAAGACATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K018966-022-036-E11C-DR03
ATGAGATCTATTATCATTAAAAGAACCCGTTTAGCAATTCATACAAGGTTCATATGTGAT
CCCAATTTACAAACACAAACGTCACACGAGCTAGTAACAAGATCCGACCCCGACAAATCG
AGTCGGACTGTACCCGAGTGTTAGGTCCAAGTTTAGCTCCTTGTCCAGATGATCATATCT
GTAATCACAATCAACACTTAACCGTAAATCCTCGTCCGTAGTCGTACCGCTCGTAGAAGC
ACAGTTTTCACAAGAAGTCTCCATCTTTAGTTTAACAGAACCGGGAAACGTGGACTTCAA
ATCTTCATTTCGGGGATGAAACGATGTCGTTTTAGGCTCGATGATCGAACCGTGAGTTTT
TGGGTCAATACCACGGTTAAGAAGCTTCCTTTTTATATGAGTGTTCCAATAATTTTTAAT
CTCATTATCGGTTCTTCCCGGTAATTTCCCAGCAATCAAAGACCATCTGAAAAAAAGACA
TTCGAAATGTAAGGAACATTGGTTTAAAACTTGGTCATTTGGGTATATTAAACCGGTTAT
TTATTTGGTTTTGTCAAATTGGATCTGCTTGTATTTTTATTATTTTTTTCTCATATTGGT
TTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
   
Sequence Name 16-K018966-022-036-E11C-8409
GenBank Accession LN493233 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K018966-022-036-E11C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAAAACCAATATGAGAAAAAAATAATAA
AAATACAAGCAGATCCAATTTGACAAAACCAAATAAATAACCGGTTTAATATACCCAAAT
GACCAAGTTTTAAACCAATGTTCCTTACATTTCGAATGTCTTTTTTTCAGATGGTCTTTG
ATTGCTGGGAAATTACCGGGAAGAACCGATAATGAGATTAAAAATTATTGGAACACTCAT
ATAAAAAGGAAGCTTCTTAACCGTGGTATTGACCCAAAAACTCACGGTTCGATCATCGAG
CCTAAAACGACATCGTTTCATCCCCGAAATGAAGATTTGAAGTCCACGTTTCCCGGTTCT
GTTAAACTAAAGATGGAGACTTCTTGTGAAAACTGTGCTTCTACGAGCGGTACGACTACG
GACGAGGATTTACGGTTAAGTGTTGATTGTGATTACAGATATGATCATCTGGACAAGGAG
CTAAACTTGGACCTAACACTCGGGTACAGTCCGACTCGATTTGTCGGGGTCGGATCTTGT
TACTAGCTCGTGTGACGTTTGTGTTTGTAAATTGGGATCACATATGAACCTTGTATGAAT
TGCTAAACGGGTTCTTTTAATGATAATAAATCTCATAACAAGTTATGTCTTTTT
Primers used for PCR FL71 - 3144, show primer details
   
Sequence Name 11-K029297-022-036-E11C-3144
GenBank Accession LN490980 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K029297-022-036-E11C-3144
ATCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGG
ACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTG
ACACATATTAAACCTTACAACATTGGATCAACTAAAATATAGTCATCCATATTACATAAA
AAACCAACAAGTCTAACTTATAAGCAGAGGCCCATAAAGAAAGCCCAAACCAGGAAACAA
AACCTTTAGAGGAAGTAAATTAAAGATTCCCTTCCACCACAAAGCCACGTGTGATGTTAA
CAAACAAAGTTGAAACCGGTTGACT
   
Sequence Name 12-K029297-022-036-E11C-FL71
GenBank Accession LN491440 [GenBank]
Sequencing primer gene specific primer, FL71: 5'-CAGTCAACGTGTTTCAACTTTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K029297-022-036-E11C-FL71
GAATCTTTAATTTACTTCCTCTAAAGGTTTTGTTTCCTGGTTTGGGCTTTCTTTATGGGC
CTCTGCTTATAAGTTAGACTTGTTGGTTTTTTATGTAATATGGATGACTATATTTTAGTT
GATCCAATGTTGTAAGGTTTAATATGTGTCAAACACTGATAGTTTAAACCGAAGGCGGGA
AACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATA
GAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATA
TCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTC
CTCGTGGGTGGGGGT
Primers used for PCR 12wp - 3144, show primer details
   
Sequence Name 29-K118496-0022-036-E11C-12wp
GenBank Accession LN499487 [GenBank]
Sequencing primer gene specific primer, 12wp: 5'-GGATTAATTTGTTTTCCACGCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K118496-0022-036-E11C-12wp
TTAGATGCGGATTAGGTTTTTTTGCTTATGGGTTGAACTATTGCAATTGTGGCTGTTTCT
CTAACTGATCTATGGAGACGAGTGCTTTTTTGTTCTTTTTTAAGGTGTTGCTGAAAGCTC
CATCGGCTTACTCTACGTCGATGTTCTTTAGATTTCAGGAATCAATTGCCTTTGGGAGAT
CTTTCCTTTCCAAATTTGCTAATGAAGTTTGGTGGATTCTCTAATTAAAAATCAATGGAC
TTCTCTTGAAGCTCCGGCGGTTTCCGGTGGTTTTAGAGTTCCCTGAGTTGCTAACCAGAA
CGGTGGTGAATGGCGACAACTAAGGCTTCTTCAGTCAACGTGTTTCAACTTTGTTTGTTA
ACATCACACGTGGCTTTGTGGTGGAAGGGAATCTTTAATTTACTTCCTCTAAAGGTTTTG
TTTCCTGGTTTGGGCTTTCTTTATGGGCCTCTGCTTATAAGTTAGACTTGTTGGTTTTTT
ATGTAATATGGATGACTATATTTTAGTTGATCCAATGTTGTAAGGTTTAATATGTGTCAA
ACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCT
CTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTG
TGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCAC
   
Sequence Name 05-K118495-0022-036-E11C-35St
GenBank Accession LN488558 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K118495-0022-036-E11C-35St
TTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGACACATATTAAAC
CTTACAACATTGGATCAACTAAAATATAGTCATCCATATTACATAAAAAACCAACAAGTC
TAACTTATAAGCAGAGGCCCATAAAGAAAGCCCAAACCAGGAAACAAAACCTTTAGAGGA
AGTAAATTAAAGATTCCCTTCCACCACAAAGCCACGTGTGATGTTAACAAACAAAGTTGA
AACACGTTGACTGAAGAAGCCTTAGTTGTCGCCATTCACCACCGTTCTGGTTAGCAACTC
AGGGAACTCTAAAACCACCGGAAACCGCCGGAGCTTCAAGAGAAGTCCATTGATTTTTAA
TTAGAGAATCCACCAAACTTCATTAGCAAATTTGGAAAGGAAAGATCTCCCAAAGGCAAT
TGATTCCTGAAATCTAAAGAACATCGACGTAGAGTAAGCCGATGGAGCTTTCAGCAACAC
CTTAAAAAAGAACAAAAAAGCACTCGTCTCCATAGATCAGTTAGAGAAACAGCCACAATT
GCAATAGTTTCAACCCATAAGCATAAAAAACCTTAATCCGCATCTTAATTTGTTCTCAAA
AAATGAAATCGATNAGCGT

Last Updated on 06.12.2018 16:11