Sequences from confirmation amplicon


Confirmation sequences for line 036F08 and hit At1g67640 are shown.
Line ID 036F08
Locus At1g67640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25352392
Primers used for PCR UF15 - 8474, show primer details
   
Sequence Name 15-K027845-022-036-F08C-UF15
GenBank Accession LN492825 [GenBank]
Sequencing primer gene specific primer, UF15: 5'-TCAACTTCTTGAATGCACTAGGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K027845-022-036-F08C-uf15
CACGTTGTGTTGGAGATACAAGCTACTATTCCTTCACACCTGAGAAACCATCAAAGATTG
CAATGTGGAAAGGAGTAGTAGTTGCATACATCGTTGTTGCCATATGCTACTTTCCCGTTG
CATTTGTTTGCTACTATATCTTTGGAAACAGTGTGGACGACAATATTCTCATGACGTTGG
AGAAACCCATCTGGCTTATCGCCATTGCTAACGCATTCGTGGTTGTCCATGTCATTGGAA
GCTACCAAATATACGCAATGCCGGTCTTTGACATGCTCGAAACCTTTTTGGTAAAGAAGA
TGATGTTCGCTCCTTCCTTCAAACTCCGCTTCATCACTCGTACTCTATATGTTGGTAAGT
TCTTATTCATATTAAATCAATTCTCCTAGATAGTAACATATGAATTTATAACATTGGATC
AATGGAACAGCTTTCACGATGTTCGTTGCCATATGCATCCCATTTTTTGGTGGATTGCTC
GGGTTTTTCGGAGGATTCGCCTTTGCCCCAACAACTTACTATGTAAGTAATTCAATACAC
TAATTAAGCCTATTCATTTGTTTGAAAATTCTAGTAACTCAACTTGATCTACCTTTTGTA
TTGTACTATGTTTAGCTACCATGATGCATGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATNTCGACGTGT
   
Sequence Name 14-K027845-022-036-F08C-8409
GenBank Accession LN492360 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K027845-022-036-F08C-8409
TTTACATGCATCATGGGTAGCTAAACATAGTACAATACAAAAGGTAGATCAAGTTGAGTT
ACTAGAATTTTCAAACAAATGAATAGGCTTAATTAGTGTATTGAATTACTTACATAGTAA
GTTGTTGGGGCAAAGGCGAATCCTCCGAAAAACCCGAGCAATCCACCAAAAAATGGGATG
CATATGGCAACGAACATCGTGAAAGCTGTTCCATTGATCCAATGTTATAAATTCATATGT
TACTATCTAGGAGAATTGATTTAATATGAATAAGAACTTACCAACATATAGAGTACGAGT
GATGAAGCGGAGTTTGAAGGAAGGAGCGAACATCATCTTCTTTACCAAAAAGGTTTCGAG
CATGTCAAAGACCGGCATTGCGTATATTTGGTAGCTTCCAATGACATGGACAACCACGAA
TGCGTTAGCAATGGCGATAAGCCAGATGGGTTTCTCCAACGTCATGAGAATATTGTCGTC
CACACTGTTTCCAAAGATATAGTAGCAAACAAATGCAACGGGAAAGTAGCATATGGCAAC
AACGATGTATGCAACTACTACTCCTTTCCACATTGCAATCTTTGATGGTTTCTCAGGTGT
TGAAGGAATAGTAGCTTGTATCTCCAACACAACGTTGTGACCTGCATACGCAAATGCCAC
ATCTCCTA

Last Updated on 06.12.2018 16:11