Sequences from confirmation amplicon


Confirmation sequences for line 036F08 and hit At4g25350 are shown.
Line ID 036F08
Locus At4g25350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12964674
Primers used for PCR YP05 - 8474, show primer details
   
Sequence Name 54-K020068-022-036-F08C-8409
GenBank Accession LN508337 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K020068-022-036-F08C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATACATGTAAAATCTACAAATCCAA
ACAAAAGATTACAGATTAGTCACCGAACTTATCCCCCTCTTTCTTCTCGTAGTAAGTTCC
TTCTATCTCTCTCTATTTCTCAGTACATTTTACACAACTCAATCTTTGTTTTTAGTAAGA
CTCGTTTCTCTGCATATTGCAGTTAGTGATTATCATTGTTCTCTGCCCCTTTAACATTTT
GTATCGGTCAAGCCGATTCTTCTTCCTCTCGGTTCTGTTCCGCTGCATTGCTGCACCATT
TTACGCGGTACCAACAACATATCCTCCTCTCCATTCATCTGAAGCATAAACAGAAATGTT
AAGAAGAATGTCTTCCATTTTTGCAGGTGCATCTTCCGGACTTTTTCTTGGGAGATCAAC
TAACAAGCCAGGTTCGAGAAAATCTATACTCGATAAGATAGCTTCATACTTATTTTGATC
GATGATTTCTGTTTAAACAATCAATGCTAGGTTCAAGCACTGAGGAGTCTAGAGTTCTAC
ATATGCTACTATGGTTTTGGAGATTTCAGATATAGACGACGAAACACCTGCACATCAAAT
ATTGGATTCAGAACATTCTACTTCATCGTCGCCGTTATACCTTACTGGTTACGGTTTCTT
CAGTGCATCCGAA
   
Sequence Name 53-K020068-022-036-F08C-YP05
GenBank Accession LN508005 [GenBank]
Sequencing primer gene specific primer, YP05: 5'-GATCTCTGTCTTCCACCATTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K020068-022-036-F08C-YP05
CCGTAACCAGTAAGGTATAACGGCGACGATGAAGTAGAATGTTCTGAATCCAATATTTGA
TGTGCAGGTGTTTCGTCGTCTATATCTGAAATCTCCAAAACCATAGTAGCATATGTAGAA
CTCTAGACTCCTCAGTGCTTGAACCTAGCATTGATTGTTTAAATCAGAAATCATCGATCA
AAATAAGTATGAAGCTATCTTATCGAGTATAGATTTTCTCGAACCTGGCTTGTTAGTTGA
TCTCCCAAGAAAAAGTCCGGAAGATGCACCTGCAAAAATGGAAGACATTCTTCTTAACAT
TTCTGTTTATGCTTCAGATGAATGGAGAGGAGGATATGTTGTTGGTACCGCGTAAAATGG
TGCAGCAATGCAGCGGAACAGAACCGAGAGGAAGAAGAATCGGCTTGACCGATACAAAAT
GTTAAAGGGGCAGAGAACAATGATAATCACTAACTGCAATATGCAGAGAAACGAGTCTTA
CTAAAAACCAGATTGAGTGTGTAAAATGTACTGAGAAATAGAGAGAGATAGAAGGAACTT
ACTACGAGAAGAAAGAGGGGG

Last Updated on 06.12.2018 16:11