Sequences from confirmation amplicon


Confirmation sequences for line 036G04 and hit At5g07870 are shown.
Line ID 036G04
Locus At5g07870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2513889
Primers used for PCR 27mm - 8474, show primer details
   
Sequence Name 65-K114263-0022-036-G04C-27mm
GenBank Accession LN511452 [GenBank]
Sequencing primer gene specific primer, 27mm: 5'-AAGAGAGATTGAACAACCAAAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K114263-0022-036-G04C-27mm
ATCTGATTCGCATGGATAAATCTAGCACCAGGACTATTATTACAATCCACAAACACAGAG
TGAGATCTTGAATTGTCGGTTTTCAAGTAGAGAGACGACCTGCAAGAGGATAGAAATGGA
CAAGTGCTATGGCTAGTGAGTCTTTAAGCTTCTGCAGCAAAAGTCTCCATGAACTCTTTT
GGTTTGACAGATTCAGAAAATGATGGTTTGAGAAAAAGAAGACCCTTTTGGATGTAATGG
ATTGAGAGCATTATATGATCCGTTGGTGAAAAATGGTAAGGTTCTTTCCATTTTTGAGGG
ATGGTTTTGGGTTTGACGAAGCATTTTGAGATTATCCATTTAGCGTGTAAATGGCATCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCNNTTCNNAAATGTAGATATCCG
   
Sequence Name 22-K114262-0022-036-G04C-8409
GenBank Accession LN496212 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K114262-0022-036-G04C-8409
GCTAAATGGATAATCTCAAATGCTTCGTCAAACCCAAAACCATCCCTCAAAAATGGAAAG
AACCTTACCATTTTTCACCAACGGATCATATAATGCTCTCAATCCATTACATCCAAAAGG
GTCTTCTTTTTCTCAAACCATCATTTTCTGAATCTGTCAAACCAAAAGAGTTCATGGAGA
CTTTGCTGCAGAAGCTTAAAGACTCACTAGCCATAGCACTTGTCCATTTCTATCCTCTTG
CAGGTCGTCTCTCTACTTTGAAAACCGACAATTCAAGATCTCACTCTGTGTTTGTGGATT
GTAATAATAGTCCTGGTGCTAGATTTATCCATGCTGAATCAGATCTAAGTGTGTCAGACA
TTCTTGGATCCACATATGTTCCTTTGGTTGTTCAATCTCTC

Last Updated on 06.12.2018 16:11