Sequences from confirmation amplicon


Confirmation sequences for line 036H01 and hit At3g58010 are shown.
Line ID 036H01
Locus At3g58010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21477251
Primers used for PCR 02uw - 8474, show primer details
   
Sequence Name 24-K118578-0022-036-H01C-02uw
GenBank Accession LN497197 [GenBank]
Sequencing primer gene specific primer, 02uw: 5'-CCACTCAAAACTCCATCTACCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K118578-0022-036-H01C-02uw
CACCTTACAGCTTAAAAACTTGAAGCCCAAAATCGAGCAACATACGTGGTTGGTCTAGGA
GTTGGATTAAGCCGCTCAAGATTCGTTATCCTTTCATTGACATCAATCTTTTGCATATCA
GTGATCTTCCCTGTAACTAGAGCCTCAAATCCTCCAGCATCATTGAACTGTAATCTCAAA
TGGTTTCAGAACTCCAACAGAGAGCAATCAAATTATTCACTAAGCAATCTAATGATGATT
TCCAAAACCAGATACTAAATTCAACCTAATTTCTAACCAATCACAGTTGAAAGTTTCAAT
TTTTAAGAATTTTTCGAAGACTTACGGCGAGAATAAGCGATTCCTTAGCAGAGAGACGCT
CCATTTCTCTGGCGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
T
   
Sequence Name 08-K118577-0022-036-H01C-8409
GenBank Accession LN489938 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K118577-0022-036-H01C-8409
GCCAGAAGAAGGAGCGTTCTCTCTGCTAAGGAATCCGCTTATTTCTCGCCGTAAGTCTTC
GAAAAATTCTTAAAAATTTGAAACTTTCAACTGTGATTGGTTAGAAATTAGGTTGAATTT
AGTATCTGGTTTTGGAAATCATCATTAGATTGCTTAGTGAATAATTTGATTGCTCTCTGT
TGGAGTTCTGAAACCATTTGAGATTACAGTTCAATGATGCTGGAGGATTTGAGGCTCTAG
TTACAGGGAAGATCACTGATATGCAAAAGATTGATGTCAATGAAAGGATAACGAATCTTG
AGCGGCTTAATCCAACTCCTAGACCAACCACGTATGTTGCTCGATTTTGAGCTTCAAAGT
TTTAAGCTTTAAGGTGTTATTTGATGCTTCCTAATAGCTAGCTTTGTCAGGTCTCCTTAT
CTTGAAGGTAGATG

Last Updated on 06.12.2018 16:11