Sequences from confirmation amplicon


Confirmation sequences for line 036H12 and hit At3g52020 are shown.
Line ID 036H12
Locus At3g52020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19300424
Primers used for PCR 28mm - 3144, show primer details
   
Sequence Name 40-K114362-0022-036-H12C-28mm
GenBank Accession LN503789 [GenBank]
Sequencing primer gene specific primer, 28mm: 5'-CGGTACAAACCTTCTTCTTATGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K114362-0022-036-H12C-28mm
ATATTTATTATTAGAATCTCCCGCAAAATTTAAAAGTATTATTTACGTGTGGATTCGCAG
TGGCGAACATATTATTCTTAGAATCTCCAGTAGGAACGGGATTCTCGTACACGAACACAG
AGTCGGATTTGGAGAATCCCGGGGACATGAAAGCAGCAGCTGATAAATACATCTTCTTGG
TGAAATGGCTGGAGAGGTTTCCGGAATATAAAGGAAGAGAATTTTACATTGCTGGTGAGA
GTTACGCCGGGCACTATGTTCCACAACTCGCACAAACCATCCTTGTTCATAACAAGAACC
AAAACTTCATCAACCTACGTGGCATTTTGATTGGTAACCCGACTCTGAACGACATAGTCG
AAACAACGGGTTCGTTTGATTATTTAGTAAGCCATGCTTTACTCTCACAAGATAGTCTTC
ATTTCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACC
CTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGT
GGGATTGTGCGTCATCCCTTACGTCAGTGGAGATAT
   
Sequence Name 10-K114361-0022-036-H12C-3144
GenBank Accession LN490758 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K114361-0022-036-H12C-3144
TTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAAATGAAGACTAT
CTTGTGAGAGTAAAGCATGGCTTACTAAATAATCAAACGAACCCGTTGTTTCGACTATGT
CGTTCAGAGTCGGGTTACCAATCAAAATGCCACGTAGGTTGATGAAGTTTTGGTTCTTGT
TATGAACAAGGATGGTTTGTGCGAGTTGTGGAACATAGTGCCCGGCGTAACTCTCACCAG
CAATGTAAAATTCTCTTCCTTTATATTCCGGAAACCTCTCCAGCCATTTCACCAAGAAGA
TGTATTTATCAGCTGCTGCTTTCATGTCCCCGGGATTCTCCAAATCCGACTCTGTGTTCG
TGTACGAGAATCCCGTTCCTACTGGAGATTCTAAGAATAATATGTTCGCCACTGCGAATC
CACACGTAAATAATACTTTTAAATTTTGCGGGAGATTCTAATAATAAATATAGTACTATC
CAAAAAAAAGAAGATAGACGAAAGATTCCATAAGAAGAAGTTTTGTAC

Last Updated on 06.12.2018 16:11