Sequences from confirmation amplicon


Confirmation sequences for line 037A05 and hit At5g44590 are shown.
Line ID 037A05
Locus At5g44590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17988993
Primers used for PCR CW18 - 8474, show primer details
   
Sequence Name 39-K104125-0022-037-A05C-CW18
GenBank Accession LN503335 [GenBank]
Sequencing primer gene specific primer, CW18: 5'-CAATTGGCAAAACAAATCATACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K104125-0022-037-A05C-CW18
GTGAGTTTCTGATCAATCTTTTGGGCAAACTCTTGATGTTAAGATGCATTCAAAGCACCG
AGTTGCAAAGGGACCGTCTCGCAAAGCTTCCATAGCTTCACTGATTCGCTTAAACGGACT
GGAACCTTACTAGACCAGTCACTAAGATCAGGTAAGCCTCCTGTTAATGCCACAAAGTTG
AGACCTTCTGAATCTGGTGTTCTCTTCCAACTAACCTGCAACAATAAATAATCGCGATGT
TTTTCTTTTTATCTTAAACGCGTAGTTAACAAAGAAATGTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 55-K104126-0022-037-A05C-8409
GenBank Accession LN508795 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K104126-0022-037-A05C-8409
TTACACANTTTCTTTGTTAACTACGCGTTTAAGATAAAAAGAAAAACATCGCGATTATTT
ATTGTTGCAGGTTAGTTGGAAGAGAACACCAGATTCAGAAGGTCTCAACTTTGTGGCATT
AACAGGAGGCTTACCTGATCTTAGTGACTGGTCTAGTAAGGTTCCAGTCCGTTTAAGCGA
ATCAGTGAAGCTATGGAAGCTTTGCGAGACGGTCCCTTTGCAACTCGGTGCTTTGAATGC
ATCTTAACATCAAGAGTTTTGCCAAAAGATTTGATCAGAAACTCACATGTGGATAGTGAA
AGTTGTGTATGATTTGTTTTGCCAATT

Last Updated on 06.12.2018 16:11