Sequences from confirmation amplicon


Confirmation sequences for line 037A07 and hit At3g14350 are shown.
Line ID 037A07
Locus At3g14350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4784396
Primers used for PCR DC01 - 8474, show primer details
   
Sequence Name 15-K017714-022-037-A07C-DC01
GenBank Accession LN492752 [GenBank]
Sequencing primer gene specific primer, DC01: 5'-TGGAGGAACAAAAGAGAGAGAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K017714-022-037-A07C-DC01
GAATTCTGAGAAGTTTAGTCTATACTATATATACCTCGTTTGCAGTGGGAAGGAAGCTAG
CGAGACCTGAGTCTGAGAGGTGTGGATTCAGCTCTGAGTCAAGTAAAATATTTGCTGATT
TAATGTTCTTGTGGACTATAGATGGTGAGCAAACTTCATGCAAGTACCTGCAACAACAAC
AATACTTTCCATGAGACTCTGCTGCATGCTATTATATTGAGCAATAATGTGATTGAGATG
TTTAAAGACTTACTCCAATGCACGTGCAGTGCCAAGAGCGATCTTGACACGGGGATTCCA
TATCAACGGTTTGCTTTCCTCTTCTGCAAGATGTAAAAAGTCATGCAATGAGCCGTTTCT
ATGGAACTCATAGACCACCAAGTGTTGCCCGTGTTCAGAACAGTAACCATCAAGCTTTGT
GACATTTTCGTGATCCAAATGCGCTATTTTCGATACAATTTCGGTAAAATCATCAGCAGT
ATCCGTGGGAAGCGCAGATGAGTCGATTTTCTTCACAGCAAGTACCTAGTAGATCAAAGA
AAATATCTCAGTAGCAGAATATTATGACATTGTGCTCAGTTTGAATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCT
   
Sequence Name 16-K017714-022-037-A07C-8409
GenBank Accession LN493219 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K017714-022-037-A07C-8409
ATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATTCAAACTGAGCACAA
TGTCATAATATTCTGCTACTGAGATATTTTCTTTGATCTACTAGGTACTTGCTGTGAAGA
AAATCGACTCATCTGCGCTTCCCACGGATACTGCTGATGATTTTACCGAAATTGTATCGA
AAATAGCGCATTTGGATCACGAAAATGTCACAAAGCTTGATGGTTACTGTTCTGAACACG
GGCAACACTTGGTGGTCTATGAGTTCCATAGAAACGGCTCATTGCATGACTTTTTACATC
TTGCAGAAGAGGAAAGCAAACCGTTGATATGGAATCCCCGTGTCAAGATCGCTCTTGGCA
CTGCACGTGCATTGGAGTAAGTCTTTAAACATCTCAATCACATTATTGCTCAATATAATA
GCATGCAGCAGAGTCTCATGGAAAGTATTGTTGTTGTTGCAGGTACTTGCATGAAGTTTG
CTCACCATCTATAGTCCACAAGAACATTAAATCAGCAAATATTTTACTTGACTCAGAGCT
GAATCCACACCTCTCAGACTCAGGTCTCGCTAGCTTCCTTCCCACTGCAAACGAGGTATA
TATAGTATAGACTAAACTTCTCAGAATTCTAGTATCTCTCTCTCTCTT
Primers used for PCR FL73 - 3144, show primer details
   
Sequence Name 27-K029299-022-037-A07C-3144
GenBank Accession LN498250 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K029299-022-037-A07C-3144
ATCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGA
CAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGA
GTCAAACTGACAGCAATGAGATTTGCTGATCCTTCGCTTTCCATCTTCAAACTGAGCTCT
GTATACTCTTCCAAATGTTCCTTCACCGAGAAGATTATCTACGCTGAAACTGTTGGTAGC
TACTTGAAGATCTGAAACCGTATATGTGTTCACATTTGAAGGAACAACAACAGCAGCTTT
CTTTGCAACAATAGGTTTTCTCATTGTTGAATCATCGTCATCAAACGACTTATGTCGCTC
AGATGGTGGAGGACGTAGATTCATCGACAATGACGTATCCAGTTTCTTTGTCTCAACCAA
TGGTGGATTCTGTACAGACTTATTCTCTGCAACCAGATGATTACATAGTTAGAAAACCAT
AAGATTATTTGATGTATAATACTTTTTGACAATAAAAAAAGTTTAATGAGTTATTACCTT
GATGAAAGTCATTGGACGC
   
Sequence Name 28-K029299-022-037-A07C-FL73
GenBank Accession LN498713 [GenBank]
Sequencing primer gene specific primer, FL73: 5'-CAACCCATTATACTAGCGTCCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K029299-022-037-A07C-FL73
CTCATTAACTTTTTTATTGTCAAAAAGTATTATANCATCAAATAATCTTATGGGTTTTCT
AACTATGTAATCATCTGGTTGCAGAGAATAAGTCTGTACAGAATCCACCATTGGTTGAGA
CAAAGAAACTGGATACGTCATTGTCGATGAATCTACGTCCTCCACCATCTGAGCGACATA
AGTCGTTTGATGACGATGATTCAACAATGAGAAAACCTATTGTTGCAAAGAAAGCTGCTG
TTGTTGTTCCTTCAAATGTGAACACATATACGGTTTCAGATCTTCAAGTAGCTACCAACA
GTTTCAGCGTAGATAATCTTCTCGGTGAAGGAACATTTGGAAGAGTATACAGAGCTCAGT
TTGAAGATGGAAAGCGAAGGATCAGCAAATCTCATTGCTGTCAGTTTGACTCAAACACTG
ATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAA
ATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTC
ATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAAC
GTCTTCTTTTTCCACGATGCTCCTCGTGGGTG

Last Updated on 06.12.2018 16:11