Sequences from confirmation amplicon


Confirmation sequences for line 038B09 and hit At5g15802 are shown.
Line ID 038B09
Locus At5g15802 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5157198
Primers used for PCR AM15 - 8474, show primer details
   
Sequence Name 02-K105747-0022-038-B09C-AM15
GenBank Accession LN487035 [GenBank]
Sequencing primer gene specific primer, AM15: 5'-CCACTGATCTTGTGTTTGATGAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K105747-0022-038-B09C-AM15
CTGTTCCTTTGCAACGGCCACAAGCTACATTCTTAAGATCTCCACATTTTCGACACATGC
CAAGAGATCTGCAAAACTTGTGTTTAGAAACATATGATGATGGCAAGTTATAGATTCAAA
AACAAGTCTAATGTCATTCAGCCATGGACATACAACACTCTAGAACATTTCTTATAGCCG
TCAGCAACCACAATGAACAGTCTTAAGATCATGTCTCATTACAAAGGTCAGATTTTCAGA
ATCCATAATCAAGATCAAGAATGATTCAATACCTGAATATTTCAAAAATCAAAAGATCTT
TCAGTGAGGAATACAGAATTTCAGGAAAACCATTACTTTCACATGGTGTGAACTCAGAAA
AAAAATGTAATCCATTGGAGATTGAAACTAAAAACATAACTCAACAATGGATTTTTTTTT
TCATTCAGAATCTATCCTTCCAAACTACAGATCACAAAGAAACTCTTAAGGATGAAAAAA
TAGTTTAAATCAAACCCTCTTTGATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAG
   
Sequence Name 18-K105748-0022-038-B09C-8409
GenBank Accession LN494306 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K105748-0022-038-B09C-8409
GAGCCATTTACAATCAAGAGGGTTTGATTTAAACTATTTTTTCATCCTTAAGAGTTTCTT
TGTGATCTGTAGTTTGGAAGGATAGATTCTGAATGAAAAAAAAAATCCATTGTTGAGTTA
TGTTTTTAGTTTCAATCTCCAATGGATTACATTTTTTTTCTGAGTTCACACCATGTGAAA
GTAATGGTTTTCCTGAAATTCTGTATTCCTCACTGAAAGATCTTTTGATTTTTGAAATAT
TCAGGTATTGAATCATTCTTGATCTTGATTATGGATTCTGAAAATCTGACCTTTGTAATG
AGACATGATCTTAAGACTGTTCATTGTGGTTGCTGACGGCTATAAAAAATGTTCTAGAGT
GTTGTATGTCCATGGCTGAATGACATTAGACTTGTTTTTGAATCTATAACTTGCCATCAT
CATATGTTTCTAAACACAAGTTTTGCAAATCTCTTGGCATGTGTCGAAAATGTGGAGATC
TTAAGAATGTAGCTTGTGGCCGTTGCAAAGGAACAGGAACAATCAAATCAGGAGGATTCT
TTGGTTTCAGTGACTCATCAAACACAGAAAACCAGTGG

Last Updated on 06.12.2018 16:11