Sequences from confirmation amplicon


Confirmation sequences for line 039A04 and hit At3g61420 are shown.
Line ID 039A04
Locus At3g61420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22727305
Primers used for PCR 02ra - 8474, show primer details
   
Sequence Name 27-K115914-0022-039-A04C-02ra
GenBank Accession LN498539 [GenBank]
Sequencing primer gene specific primer, 02ra: 5'-TGACCGATTCAGTGACTACATAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K115914-0022-039-A04C-02ra
GTTCTTAACCACTGTTTTGCCACCCAAAAAAAAAACAATTGGGGTATTGAGAGGTTCCTT
GTAGCACAAGCAACATTCTTGGCCAAATCCCGCTAATGATGCTTCTCACTCAAGTTCTTT
AAAAATCTTATCTATTACACTACAAAATCTAAATGTTCAGTCCAATGTTTAATTTTTTGT
CAAAGGAAATCTCTTTCTTTCCAAGTACAAAACGTTCTCTATGTAATCTTAGCGGTTCTT
CTCTGCAAGTCCAATTCGTAGTGTTGGAACGCAGCATCAAAAGCCTGGTAAATGGCTTCT
CTGTAATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAAATG
   
Sequence Name 01-K115913-0022-039-A04C-8409
GenBank Accession LN486715 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K115913-0022-039-A04C-8409
TTTACATTGAAATATCCTGATTACGAGAAGCCATTTACCAGGCTCTTGATGCTGCGTTCC
AACACTACGAATCGGACTTGCAGAGAAGAACCGCTAAGATTACATAGAGAACGTTTTGTA
CTTGGAAAGAAAGAGATTTCCTTCGACAAAAAATTAAACATTGGACTGAACATTTAGATT
TTGTAGTGTAATAGATAAGATTCTTAAAGAACTTGAGTGAGAAGCATCATTAGCGGGATT
TGGCCAAGAATGTTGCTTGTGCTACAAGGAACCTCTCAATACCCCAATTGTTTTTTTTTT
GGGTGGCAAAACAGTTGGTTAAAAACTTGATTGAAAAGCATTAGCGGGGTTTGGTTAAAA
ATGTTGCTTGAGCTATGTAG

Last Updated on 06.12.2018 16:11