Sequences from confirmation amplicon


Confirmation sequences for line 040A12 and hit At1g09070 are shown.
Line ID 040A12
Locus At1g09070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2927866
Primers used for PCR HM38 - 8474, show primer details
   
Sequence Name 15-K032413-022-040-A12C-8409
GenBank Accession LN492860 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K032413-022-040-A12C-8409
TCATTTATGGACATGATGGAAACGACGGCGTACAAGTCTTGTTTACCGATCAATTGAACG
TCTTTGAGATCCTCCGCAGATATAATCGTCAGATCCAATGATCTACACTCCATGGTTACT
CGGATCGAAATTGAATGAAATAAACTTGAAATTTGCTCTGAGAATGTTTTTTATGATGAG
GAAAAGAAAAGTGGAGAGGGCAAAGGATATATATGCATTTATGATAAAGTCGGTTTAAGG
AACAAAGAGTTTTTTTTTTACACACGGGAGGGACACGCACGAGATTTTTTTTGTTAAGGA
CGCGGTTTCTTTAAATGCAAATAGCTTTTTTCCTTCTAATATTAGTTTCTTCTTATCTGA
AGTTTAATTATCTTGCTGTTTTTAGTAAATTATTATTTGACCATGGTTCAAATAATAAAC
TCATGTGTATCTAAAAGTTTAGTGATAATGGCAATGTAAAC
   
Sequence Name 15-K032413-022-040-A12C-HM38
GenBank Accession LN492861 [GenBank]
Sequencing primer gene specific primer, HM38: 5'-TATCTTTGAACGTTTACATTGCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K032413-022-040-A12C-HM38
TATTTGAACCATGGTCAAATAATAATTTACTAAAAACAGCACGATAATTAAACTTCAGAT
AAGAAGAAACTAATATTAGAAGGAAAAAAGCTATTTGCATTTAAAGAAACCGCGTCCTTA
ACAAAAAAAATCTCGTGCGTGTCCCTCCCGTGTGTAAAAAAAAAACTCTTTGTTCCTTAA
ACCGACTTTATCATAAATGCATATATATCCTTTGCCCTCTCCACTTTTCTTTTCCTCATC
ATAAAAAACATTCTCAGAGCAAATTTCAAGTTTATTTCATTCAATTTCGATCCGAGTAAC
CATGGAGTGTAGATCATTGGATCTGACGATTATATCTGCGGAGGATCTCAAAGACGTTCA
ATTGATCGGTAAACAAGACTTGTACGCCGTCGTTTCCATCATGTCCATAAATGAGTAAAT
GGCTATATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACT
Primers used for PCR FN44 - 3144, show primer details
   
Sequence Name 25-K032734-022-040-A12C-3144
GenBank Accession LN497333 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K032734-022-040-A12C-3144
TCCACATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTAAAC
AAGATTTCATGGTTGACAGGATATATTCAATTGTAAATGGCTTCTGCTAGGACGAAGCAG
AAGACAAAGGTTGATAAAGATTGCGGCACCAAACCTAAATGGAAGCATCAAATGAAGCTC
ACCGTCGATGACGCAGCGGCGCGTGACAATCGTCTTACTCTTGTTTTCGAGATCGTGGCG
GATCGTCCCATCGCTGGTGATAAACCTGTCGGTGAGGTTAGCGTTCCGGTGAAGGAGCTT
TTGGATCAGAATAAAGGTGACGAGGAGAAAACGGTTACTTACGCCGTGAGGTTGCCTAAC
GGGAAGGCGAAAGGATCTCTCAAATTCTCGTTCAAATTTGGGAAAAAAGTAT
   
Sequence Name 57-K032734-022-040-A12C-FN44
GenBank Accession LN509326 [GenBank]
Sequencing primer gene specific primer, FN44: 5'-TATACTTTTCCCCAAATTTGAACG-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K032734-022-040-A12C-FN44
TCCGTTAGGCACCTCACGGCGTAAGTAACCGTTTTCTCCTCGTCACCTTTATTCTGATCC
AAAAGCTCCTTCACCGGAACGCTAACCTCACCGACAGGTTTATCACCAGCGATGGGACGA
TCCGCCACGATCTCGAAAACAAGAGTAAGACGATTGTCACGCGCCGCTGCGTCATCGACG
GTGAGCTTCATTTGATGCTTCCATTTAGGTTTGGTGCCGCAATCTTTATCAACCTTTGTC
TTCTGCTTCGTCCTAGCAGAAGCCATTTACAATTGAATATATCCTGTCAACCATGAAATC
TTGTTTAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTAC
CCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAG
TGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAA
GACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGG

Last Updated on 06.12.2018 16:11