Sequences from confirmation amplicon


Confirmation sequences for line 040D08 and hit At1g51960 are shown.
Line ID 040D08
Locus At1g51960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19311316
Primers used for PCR CW15 - 8474, show primer details
   
Sequence Name 40-K104125-0022-040-D08C-CW15
GenBank Accession LN503690 [GenBank]
Sequencing primer gene specific primer, CW15: 5'-ATTTTGGCAAATGGTTTTGTTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K104125-0022-040-D08C-CW15
GTTTGAACGTTTTTCTTCATAGATTACAGTTTTAGCAAATGTGAATCATTAAAAGTGGAA
TAGTTGGTTTGAAAACAATTGTCAATTTCATTTTTTTTTTGGTTTTATGGTTAGGCGAGG
AAAGCATTAAAAGCTTTGAAAGGTATAGTGAAGCTACAAGCATTAGTGAGAGGATACTTA
GTAAGGAAACGCGCGGCCGCAATGTTGCAAAGCATACAAACTTTGATCAAAGTCCAAACC
GCTTTGCGATCAAAACGCATCATTCGCAGCCTCAACAAAAAGTACAACAACATGTTTCAA
CCTCGACAATCCTTTGTAAAGAACTATTCTCATTTCCTTTGGCTCTTTTTTTTTCTTTAA
GCCAAAACAAAACTTAAAGTGTGTCCCCTGTTTGTAGGATAATTTTGATGAACCAACGTT
CGATGACGGAAAAACAAAGATGGTAAAGAAGGACAATGGATACTTGAGAAGATCAAGTTC
AAGATCAAGATCTAGACAAGTGCACAATGTTGTTTCAATGTCTGACTATAAAGGCAATTT
TGTAAATTTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACTTGGATCACCAATGAG
TATAATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAAATTTC
   
Sequence Name 56-K104126-0022-040-D08C-8409
GenBank Accession LN509085 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K104126-0022-040-D08C-8409
TGAAGCCATTTACAATTGAACATTTACAAAATCGCCTTCATAGTCAGACATTGAAACAAC
ATTGTGCACTTGTCTAGATCTTGATCTTGAACTTGATCTTCTCATGTATCTATCGTCCTT
CTCTACAATCTTTGTTCTTCTGTCATCGAACGTTGCTTCATCAAACTTATCCTACAAACA
GAGGACACACTTTAAGTCTTGTTTTGGCTTAAAGAAAAAAAGAGAGCCAATGGAAATGAG
AATAGTTCTTTACAAAGGATTGTCGAGGTTGAAACATGTTGTTGTACTCTTTGTTGAGGC
TGCGATTGATGCGTTTTGATCGCATAGCGGTTTGGACTCTGATCAAAGTTTGTATGCTCT
GCAACATTGCGGCCGCGCGTTTCCTTACTAAGTATCCTCTCACTAATGCTTGTAGCTTCA
CTATACCTTTCAAAGCTCTTAATGCTTTCCTCGCCTAACCATAAAACCAAAAAAAAAATG
AAATTGACAATTGTTTTCAAACCAACTATTCCACTTTTAATGATTCACATTTGCTA

Last Updated on 06.12.2018 16:11