Sequences from confirmation amplicon


Confirmation sequences for line 040G11 and hit At2g32990 are shown.
Line ID 040G11
Locus At2g32990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14004834
Primers used for PCR 05lm - 8474, show primer details
   
Sequence Name 46-K114240-0022-040-G11C-05lm
GenBank Accession LN505934 [GenBank]
Sequencing primer gene specific primer, 05lm: 5'-GAAAAGTTATTATGCCTCGGTGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K114240-0022-040-G11C-05lm
CTTATCGAGCCACCGACATGAGCACTATATGAGTTATGTGGTTGACATGGCTCACCAACT
AGGTGGCCTTTCTTGGGCCATGTCCGAGTTTAGCTGGGACGTCAAGTTCGCCGGTGTTCA
ACTCCTAGCTTCCATGGTTAGTACTTACATATTGTTCAATTTTGTGTGTGGTAAAAGTGA
AATATTTATAGCACTAACCTCTTAGTAGTCTAATTTAAGATTTAAGTAGAATCTTATAAT
TGCCTTATGTGAAGTGATTATCAAGTTTAAATATTCTCACGTCGAATTACCAAAAGTAAG
GTCAGCAATCTACATGAAAAACAACAATATGATGTCCGGGAAATCTACATGGATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGATGTCCG
   
Sequence Name 22-K114239-0022-040-G11C-8409
GenBank Accession LN496211 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K114239-0022-040-G11C-8409
GTTTTTATGTAGATTGCTGACCTTACTTTTGGTAATTCGACGTGAGAATATTTAAACTTG
ATAATCACTTCACATAAGGCAATTATAAGATTCTACTTAAATCTTAAATTAGACTACTAA
GAGGTTAGTGCTATAAATATTTCACTTTTACCACACACAAAATTGAACAATATGTAAGTA
CTAACCATGGAAGCTAGGAGTTGAACACCGGCGAACTTGACGTCCCAGCTAAACTCGGAC
ATGGCCCAAGAAAGGCCACCTAGTTGGTGAGCCATGTCAACCACATAACTCATATAGTGC
TCATTGTCGGTGGCTCGATAAAGCCAAGTGGCACCCCATAAAAGCTCGTCCATGTACCCA
CTCACCGAGGCATAAAAACTTTT

Last Updated on 06.12.2018 16:11