Sequences from confirmation amplicon


Confirmation sequences for line 042C04 and hit At1g28815 are shown.
Line ID 042C04
Locus At1g28815 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10095943
Primers used for PCR 01ny - 8474, show primer details
   
Sequence Name 28-K114669-0022-042-C04C-01ny
GenBank Accession LN498963 [GenBank]
Sequencing primer gene specific primer, 01ny: 5'-TAGAGAGGCAAGAAAGCAAGAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K114669-0022-042-C04C-01ny
CCACTCTAGAAAGGACATTTAAACTTATTTTTGTTGTAGCCTCGGTAATTGTATAAGTTA
TACACAAAGTAAATAAAATGCTGATTGTACTAGCTAATAAGACCATTCCTTAGGAAGAAA
GATTTTTCTGGAATGAGAGGAGATGATGGGGCATGGCTTGTTGTTGAGTTTCTCTTGTGA
GCTACTCACCGGTCTCTCAGGAAGAAACACTCCAGTGCCACGTGGCTTAACTCGTGCTGC
ACCTTTATCATCACCATTTGGCCTCACCATGGAACCCTTGTGGTTCAATGCATACCACAT
TCTTGGCTTCCGATCATAGTAGCTACCTACTCGATTACTCTACAGATTATGTAACAAAGA
TTGATTAATTATACATCCTTAGCATTAATAACAATACATCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 05-K114668-0022-042-C04C-8409
GenBank Accession LN488456 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K114668-0022-042-C04C-8409
AACCGGAAGAACCATTTACATTGATGATTGTTATTAATGCTAAGGATGTATAATTAATCA
ATCTTTGTTACATAATCTGTAGAGTAATCGAGTAGGTAGCTACTATGATCGGAAGCCAAG
AATGTGGTATGCATTGAACCACAAGGGTTCCATGGTGAGGCCAAATGGTGATGATAAAGG
TGCAGCACGAATTAAGCCACGTGGCACTGGAGTGTTTCTTCCTGAGAGACCGGTGAGTAG
CTCACAAGAGAAACTCAACAACAAGCCATGCCCCATCATCTCCTCTCATTCCAGAAAAAT
CTTTCTTCCTAAGGAATGGTCTTATTAGCTAGTACAATCAGCATTTTATTTACTTTGTGT
ATAACTTATACAATTACCGAGGCTACAACAAAAATAAGTTTAAATGTCCTTTTCTAGAGT
TGGCACCCTAAAGATCAGTATTGGGTATGGTTCCTCAATGATTCTTGCTTTCTGCCCTCT
C

Last Updated on 06.12.2018 16:11