Sequences from confirmation amplicon


Confirmation sequences for line 042D10 and hit At4g17565 are shown.
Line ID 042D10
Locus At4g17565 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9783592
Primers used for PCR 04wx - 8474, show primer details
   
Sequence Name 51-K118874-0022-042-D10C-04wx
GenBank Accession LN507652 [GenBank]
Sequencing primer gene specific primer, 04wx: 5'-TAAACTTCCGAAAATGCAGAAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K118874-0022-042-D10C-04wx
TTTTGGGAGTATGCAAAAATACCAAGAGTTGTCCAAACTAGAAAAGAATACCTCTTTTCC
TTTTTCCTTTTTTGTGTATGTGAAAAGATTAATGCAAAAGACGTTGATGCGCTTGCTTTA
GTATATGTATAAACACTTTTTTATTTTATCTTTATAATCAACTATCAACTTTATAATTCT
ACCACTTGAATTACAACAAATTTTCAGTTTTAACGTGTTTTCTTTGAAATTTAGCAATGG
ACCAAAGCGACAAGAACCCTAATCGGCGGCTCCGAGTGATTCCCAAGTGGTCGGAGCTTT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGGTTATTA
   
Sequence Name 11-K118873-0022-042-D10C-8409
GenBank Accession LN491343 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K118873-0022-042-D10C-8409
GCTCGACACTTGGGAATCACTCGGAGCCGCCGATTAGGGTTCTTGTCGCTTTGGTCCATT
GCTAAATTTCAAAGAAAACACGTTAAAACTGAAAATTTGTTGTAATTCAAGTGGTAGAAT
TATAAAGTTGATAGTTGATTATAAAGATAAAATAAAAAAGTGTTTATACATATACTAAAG
CAAGCGCATCAACGTCTTTTGCATTAATCTTTTCACATACACAAAAAAGGAAAAAGGAAA
AGAGGTATTCTTTTCTAGTTTGGACAACTCTTGGTATTTTTGCATTACTCCCAAAATTCT
GATAAAGTAGTACTCATGTATTCCATTTTCTGCATTTCCGGAAGTTT

Last Updated on 06.12.2018 16:11