Sequences from confirmation amplicon


Confirmation sequences for line 044H04 and hit At1g26130 are shown.
Line ID 044H04
Locus At1g26130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9036505
Primers used for PCR 01qf - 8474, show primer details
   
Sequence Name 34-K115550-0022-044-H04C-01qf
GenBank Accession LN501539 [GenBank]
Sequencing primer gene specific primer, 01qf: 5'-CTCAAGGAAGATGCCTTTCATATC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K115550-0022-044-H04C-01qf
TCCATCATGATTAAAGCAAAGCCTTAGCGTTTCCACCAGACGCTTTCAGTTGAGCCTTTC
CACTAGTTATCTGATGCAGTACATTTTCCTTTAAAGCCTGCAAAAAATAGTCAAATGGAA
TTCGTTCAGCTACATAATCACTAGTAACACTACTACATGATCACTTATACTATTACTAGT
AATATCAGTGTTCTAGAAAATTTGTATTACCGCTGCAATGGCATCTTTCTCTCCGGATTT
TTCCAACTGCTGGATTTCAGGAGTCTCCAAGTTAATTATGATCCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 07-K115549-0022-044-H04C-8409
GenBank Accession LN489410 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K115549-0022-044-H04C-8409
CCTGAATCCAGCAGTTGGAAAAATCCGGAGAGAAAGATGCCATTGCAGCGGTAATACAAA
TTTTCTAGAACACTGATATTACTAGTAATAGTATAAGTGATCATGTAGTAGTGTTACTAG
TGATTATGTAGCTGAACGAATTCCATTTGACTATTTTTTGCAGGCTTTAAAGGAAAATGT
ACTGCATCAGATAACTAGTGGAAAGGCTCAACTGAAAGCGTCTGGTGGAAACGCTAAGGC
TTTTGCTTTAATCATTGATGGGAAATCACTTGCTTATGCATTGGAGGAAGATATGAAAGG
CATCTTCCTTGA

Last Updated on 06.12.2018 16:11