Sequences from confirmation amplicon


Confirmation sequences for line 045G07 and hit At2g34030 are shown.
Line ID 045G07
Locus At2g34030 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14377279
Primers used for PCR CL49 - 8474, show primer details
   
Sequence Name 71-K033097-022-045-G07C-8409
GenBank Accession LN512495 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K033097-022-045-G07C-8409
CTGTGCAGACATGTTGAAGGTTATATCTTCCAAGTTTTTTCCTTTGGGAGTCTCTTGATT
ATCGGTGACTATTTCTTGTCCGAAGGAAGATCTAAACTCTTTGTAATATTCGAGGTCGGG
TTCTATGGTGGTATCATCTTCCCTCTTCTTACAATGTTCCCAAGAATCGCTCTTATGCTC
TGTAAGGATCCTTTCTTTCTCTTTGGTTCCCTCTAAGAAAATATCTGAAAACGTATATTC
TTTGATCTCAGCGACCGGACTATCATTGAGTAGGGATGTTGCTAGCTCCTTTATAGATGA
TAACGTTGGACTTACTGTGGGACACACTGTGTTTTCTCTCACAATTCAATGGGGAGCTTG
TGTTGTGTTTAGCATCACTGGTCCACGTTCTGACCAAGCAGTAAGAAGAACAACCTCTGT
AGGATTTCCTTAATCTATTAGTAAATTAAGGATTGGTTGCTGAATGTTCATCGAAAAATC
ATACAGGATGGATTGATCAAAAAAACTA
   
Sequence Name 23-K033097-022-045-G07C-CL49
GenBank Accession LN496456 [GenBank]
Sequencing primer gene specific primer, CL49: 5'-TTTAGTTTTTCGATCAATCCATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K033097-022-045-G07C-CL49
CACCATCCTTATTTACTAATAGATTAAGGAAATCCTACAGAGGTTGTTCTTCTTACTGCT
TGGTCAGAACGTGGACCAGTGATGCTAAACACAACACAAGCTCCCCATTGAATTGTGAGA
GAAAACACAGTGTGTCCCACAGTAAGTCCAACGTTATCATCTATAAAGGAGCTAGCAACA
TCCCTACTCAATGATAGTCCGGTCGCTGAGATCAAAGAATATACGTTTTCAGATATTTTC
TTAGAGGGAACCAAAGAGAAAGAAAGGATCCTTACAGAGCATAAGAGCGATTCTTGGGAA
CATTGTAAGAAGAGGGAAGATGATACCACCATAGAACCCGACCTCGAATATTACAAAGAG
TTTAGATCTTCCTTCGGACAAGAAATAGTCACCGATAATCAAGAGACTCCCAAAGGAAAA
AACTTGGAAGATATAACCTTCAACATTGTCTGCACATGACTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACA
Primers used for PCR CL50 - 8474, show primer details
   
Sequence Name 72-K033097-022-045-G07C-oriL
GenBank Accession LN512660 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K033097-022-045-G07C-oriL
GCCATTTACATTGAATACATGACTGATCAGGACACGAGAGCTCACACCAGAGATAATCAG
AGCTATAGAGAGAAGGGAGAAGAAGAGAGTAACACCTGCCATGGAAGTAAGATCGAAGAT
CTAATAGGGATTTGATCGGATAATTTTCCTCTGTATACAATCGCACAAGATCTTAAATAT
ACAGTAAACATAGAGGCTTTAAGGTTAATTGTAGTACGATGAAGACTGATAGGATTATGT
TTTGATAAAGCGTATGAACAAATTATTAAGATGATGAATGGTACGTTTTTAAAAACATAT
CCCCTTGTAATCTTTATACGTAACTAAGAGCTTTGACCATTCATTTGGACAAGACAGTGT
GTTTGTGATTAAGAGACACCCTAATGGAGAGTCATTGCTAACTCTAAAGCGTGCCATGGA
AGTAATCTTGGGATGAAGGAGACATGCAATGGACCTCTCATGTTATGTAGCTATAGACTA
GAAAGATTGTATAACTAAGGTCATAAATGCTCGAAATATCTTTTTTTCTAATGCTCGAAA
TATCTTTAGTGAACAGAGAAAAACACAGATGATCTATTCTGATATGAGAAGAGAAGTCTC
CAAACTCTACTAAGTACCAAAATCAAAACTTGTACTGACAGACAGACACAGAAA
   
Sequence Name 24-K033097-022-045-G07C-CL50
GenBank Accession LN496897 [GenBank]
Sequencing primer gene specific primer, CL50: 5'-CCATGACTGTGTTTCTGTTTCTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K033097-022-045-G07C-CL50
GATTTTGGTATTAGTAGAGTTTGGAGACTTCTCTTCTCATATCAGAATAGATCATCTGTG
TTTTTCTCTGTTCACTAAAGATATTTCGAGCATTAGAAAAAAAGATATTTCGAGCATTTA
TGACCTTAGTTATACAATCTTTCTAGTCTATAGCTACATAACATGAGAGGTCCATTGCAT
GTCTCCTTCATCCCAAGATTACTTCCATGGCACGCTTTAGAGTTAGCAATGACTCTCCAT
TAGGGTGTCTCTTAATCACAAACACACTGTCTTGTCCAAATGAATGGTCAAAGCTCTTAG
TTACGTATAAAGATTACAAGGGGATATGTTTTTAAAAACGTACCATTCATCATCTTAATA
ATTTGTTCATACGCTTTATCAAAACATAATCCTATCAGTCTTCATCGTACTACAATTAAC
CTTAAAGCCTCTATGTTTACTGTATATTTAAGATCTTGTGCGATTGTATACAGAGGAAAA
TTATCCGATCAAATCCCTATTAGATCTTCGATCTTACTTCCATGGCAGGTGTTACTCTCT
TCTTCTCCCTTCTCTCTATAGCTCTGATTATCTCTGGTGTGAGCTCTCGTGTCCTGATCA
GTCATGTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA

Last Updated on 06.12.2018 16:11