Sequences from confirmation amplicon


Confirmation sequences for line 046E12 and hit At5g24380 are shown.
Line ID 046E12
Locus At5g24380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8325014
Primers used for PCR MJ15 - 8474, show primer details
   
Sequence Name 14-K021339-022-046-E12C-8409
GenBank Accession LN492311 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K021339-022-046-E12C-8409
GTAGATTTGGGTTATTGAATGTAACCCAATTGAATATGAAGCCTATATATTGAATGTAGA
TTTCTTATATAATCCTTCAAATGTTTAAACATGTTTGATTGAAGAAGCTTTATCTCTTTA
TGGGATATAGATTCTACTTTGACTTCAGTATGACATATGTTGGAGCTGGGATGATTTGTT
CTCATTTGGTGAATCTGTCGTTGCTCTTCGGTGCCATCCTCTCGTGGGGAATCATGTGGC
CTCTCATAGCCCGGCTTAAAGGCGAATGGTTCCCTGCAACATTGAAAGATAATAGTATGC
AGGGCTTAAACGGCTACAAGGTCTGATTTCATCCTAGTTTTTTATATATATATCTTACAG
CTGTGGTAGCAAAAAGATGCGATAAGTTTTGTTTGTGTTGTTGCAGGTGTTTATATGCAT
TGCACTGATCTTAGGAGATGGGCTT
   
Sequence Name 15-K021339-022-046-E12C-MJ15
GenBank Accession LN492772 [GenBank]
Sequencing primer gene specific primer, MJ15: 5'-CGAAGTTATAAAGCCCATCTCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K021339-022-046-E12C-MJ15
AGTGCAATGCATATAAACACCTGCAACAACACAAACAAAACTTATCGCATCTTTTTGCTA
CCACAGCTGTAAGATATATATATAAAAAACTAGGATGAAATCAGACCTTGTAGCCGTTTA
AGCCCTGCATACTATTATCTTTCAATGTTGCAGGGAACCATTCGCCTTTAAGCCGGGCTA
TGAGAGGCCACATGATTCCCCACGAGAGGATGGCACCGAAGAGCAACGACAGATTCACCA
AATGAGAACAAATCATCCCAGCTCCAACATATGTCATACTGAAGTCAAAGTAGAATCTAT
ATCCCATAAAGAGATAAAGCTTCTTCAATCAAACATGTTTAAACATTTGAAGGATTATAT
AAGAAATCTACATTCAATATATAGGCTTCATATTCAATTGGGTTACATTCAATAACCCAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACA
TTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATT
ATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA
Primers used for PCR FL94 - 3144, show primer details
   
Sequence Name 88-K029207-022-046-E12C-3144
GenBank Accession LN514816 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K029207-022-046-E12C-3144
CCATCCACTATCCTTCGCAAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAG
AGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCAAAGCTTGTAACCCAAATGTA
GGGAACTGAGAGAATCCGCATTTTTCACCACCTGAATAGAACCATCCAAAGAATGCCCAA
AAGAAACTCAAACCAAACGATTTTATAAACCCGCGAATCTGTTTCCTGTATCGAAAACGA
CGAAAAATATCGATATATATCCCAATCAAGTAAGAAAAAACAAGATTCATCAAGAACATA
TATAGAAAACATACTTGGCTGTCTTATCTCCTTTGCTAGTATGAAATCCATTTATAAGAA
CAGCTGTTGCCGTTCCACTAGGATAAGTTAGCTTGTAGTCTATAATCATCACCTGAAATT
TATACAATTCATTCTCAGAATTTAATCACACCATTGAAATCAAAAATTTACTCTGTTTTC
ATAATTGCTAGATATCAGTACCTTTCGAAGAGGTACCAATACAACAAGTCCAATGAAGCT
AGTAACGAAGAGGAATGATGTCATCCATCCAACACCGGGCTCTTTTATACCACGAGGATT
GTTTCCTTCGGTGTTCACTCCTGTCTCCTCGTAAGTTCGTCTATTCAAACCCAACAAATA
CGATGCANATCCACCTGCAAGAGAGAT
   
Sequence Name 89-K029207-022-046-E12C-FL94
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, FL94: 5'-TGTTGCTTGTTACAGCATCTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K029207-022-046-E12C-FL94
AGTATACCATATATGTTCTTGACGAAACTA

Last Updated on 06.12.2018 16:11