Sequences from confirmation amplicon


Confirmation sequences for line 046G07 and hit At2g13680 are shown.
Line ID 046G07
Locus At2g13680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:5700378
Primers used for PCR VL39 - 8474, show primer details
   
Sequence Name 07-K024206-022-046-G07C-VL39
GenBank Accession LN489090 [GenBank]
Sequencing primer gene specific primer, VL39: 5'-TATAAGGAACAAGCAACAGATCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K024206-022-046-G07C-VL39
GTTGGTTTTAAGGGCACGGAAAATAAGGTATTCATGAAGAAAGGTAGTATAATACCTAAG
TTGTTACCTGTCACTGATTAGATCTTCCTCTCGGAAGCTGGAAATGATTTCATTCCAGAG
TTGGGAGAATTTTGCCGCTTCAGTTCTTCTTGCTGCAGTGACCTGCATACAGATATAATT
TAGTAGATGTATGACAACATTCTTTCCACAATCACTTTGTTGATAAAACGAGCATCAATC
TTGTGTAACCTCTGCAAATCTTTTTGACAAGGAAAACCCTCTTCTCCTTGTTTTATCAGA
AGGAACCAGGTACGTATTAAATGCACCAGGTAATGATTGGAACCTTGACATATGACATTA
AATGGAGGTGTGTGTTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCT
   
Sequence Name 08-K024206-022-046-G07C-8409
GenBank Accession LN489554 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K024206-022-046-G07C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATAACACACACCTCCATTTA
ATGTCATATGTCAAGGTTCCAATCATTACCTGGTGCATTTAATACGTACCTGGTTCCTTC
TGATAAAACAAGGAGAAGAGGGTTTTCCTTGTCAAAAAGATTTGCAGAGGTTACACAAGA
TTGATGCTCGTTTTATCAACAAAGTGATTGTGGAAAGAATGTTGTCATACATCTACTAAA
TTATATCTGTATGCAGGTCACTGCAGCAAGAAGAACTGAAGCGGCAAAATTCTCCCAACT
CTGGAATGAAATCATTTCCAGCTTCCGAGAGGAAGATCTAATCAGTGACAGGTAACAACT
TAGGTATTATACTACCTTTCTTCATGAATACCTTATTTTCCGTGCCTTAAAACCAACTAT
TTTTTCAGGGAGATGGATCTGTTGCTTG

Last Updated on 06.12.2018 16:11