Sequences from confirmation amplicon


Confirmation sequences for line 047B01 and hit At4g15310 are shown.
Line ID 047B01
Locus At4g15310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8739772
Primers used for PCR KM14 - 8474, show primer details
   
Sequence Name 63-K032606-022-047-B01C-8409
GenBank Accession LN510911 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K032606-022-047-B01C-8409
CCTTACATTGAATATATCCTGATGAAGTATTATTTCCACACAAATAATCGTTTAGACTTA
CCCTAATTCTTTATGTTTGTATTATAATACTCTTGATTAATCAAAGTTACCTGCCACCTC
CATGGATTAAAGGTAAGTGGATCATCATACTTTTCTTCATCAAAGTGAACTTGAGGGATG
CCAAAAAATAGCCACCCAGCAGGTAATGTATAATCTGTCCAAACCACTGACTCATGAGCA
AACTCCATATGTTTCTGTTAGTGTGTGGAAACATACACTAATCAGTCATAAGGTGTTATG
GTGAACGGTGGAAAAAGTTTGGTTAGATCTTGCCCAACCCGTATTAGACTAAACATGTGA
TGAGGGATGAGTCACCCCGACAAACCCCTAGCTAGCATGCCCTTTGTTTCTGGTGGTTAT
GGCTGAGTCATAGGTCTAGTTTAAATCCTAAAAATTGTGTCTAACAGAACTAAACTCCAC
   
Sequence Name 63-K032606-022-047-B01C-KM14
GenBank Accession LN510912 [GenBank]
Sequencing primer gene specific primer, KM14: 5'-TGATGAACCATTTTGTGGAGTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K032606-022-047-B01C-KM14
ACCTATGACTCAGCCATAACCACCAGAAACAAAGGGCATGCTAGCTAGGGGTTTGTCGGG
GTGACTCATCCCTCATCACATGTTTAGTCTAATACGGGTTGGGCAAGATCTAACCAAACT
TTTTCCACCGTTCACCATAACACCTTATGACTGATTAGTGTATGTTTCCACACACTAACA
GAAACATATGGAGTTTGCTCATGAGTCAGTGGTTTGGACAGATTATACATTACCTGCTGG
GTGGCTATTTTTTGGCATCCCTCAAGTTCACTTTGATGAAGAAAAGTATGATGATCCACT
TACCTTTAATCCATGGAGGTGGCAGGTAACTTTGATTAATCAAGAGTATTATAATACAAA
CATAAAGAATTAGGGTAAGTCTAAACGATTATTTGTGTGGAAATAATACTCATCAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTACTTCCCCCCAAAAAGG
Primers used for PCR FP18 - 8474, show primer details
   
Sequence Name 85-K035342-022-047-B01C-8409
GenBank Accession LN514499 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K035342-022-047-B01C-8409
CCGGAATGAGCATTTACAATTGAATATATCCTGGAATACATGCCGTTTGGAGCTGGCGGG
ACTCATTGTGTAGGGTCAGAGTTTGCTAAGCTTATAATTGCAATCTTGCTGCATCATTTG
TCCAGATTCAGGTTAATTAATTTAAACTAAGTGTTTATTAAACCAAATTGAAAAAATAGA
TTGTTACTTAAATGATGGGAATTATTTTTGTCAGGTGGTCATTGGATCCAAAAACAGAAG
TACTTCGAAGGTATACACTTGTGTTCCCGGCTGGATGTGTAGTCCACATCTCCAAGGAAT
CGGAGTGATGATCATTGAATTTTGGTTTGGCATTACATTTCTTGTACAATAATTAACGAT
TATTGACTTTGGCCTGG
   
Sequence Name 85-K035342-022-047-B01C-FP18
GenBank Accession LN514500 [GenBank]
Sequencing primer gene specific primer, FP18: 5'-AATGCCAGACCAAAGTCAATAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K035342-022-047-B01C-FP18
GAATGTATGCCAAACCAAAATTCAATGATCATCACTCCGATTCCTTGGAGATGTGGACTA
CACATCCAGCCGGGAACACAAGTGTATACCTTCGAAGTACTTCTGTTTTTGGATCCAATG
ACCACCTGACAAAAATAATTCCCATCATTTAAGTAACAATCTATTTTTTCAATTTGGTTT
AATAAACACTTAGTTTAAATTAATTAACCTGAATCTGGACAAATGATGCAGCAAGATTGC
AATTATAAGCTTAGCAAACTCTGACCCTACACAATGAGTCCCGCCAGCTCCAAACGGCAT
GTATTCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTACATTCACGT
Primers used for PCR FP18 - 8474, show primer details
   
Sequence Name 52-K118471-0022-047-B01C-FP18
GenBank Accession LN507960 [GenBank]
Sequencing primer gene specific primer, FP18: 5'-AATGCCAGACCAAAGTCAATAATC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K118471-0022-047-B01C-FP18
CAACAAAATTCATGATCATCACTCCCGATTCCTTGGAGATGTGGACTACACATCCAGCCG
GGAACACAAGTGTATACCTTCGAAGTACTTCTGTTTTTGGATCCAATGACCACCTGACAA
AAATAATTCCCATCATTTAAGTAACAATCTATTTTTTCAATTTGGTTTAATAAACACTTA
GTTTAAATTAATTAACCTGAATCTGGACAAATGATGCAGCAAGATTGCAATTATAAGCTT
AGCAAACTCTGACCCTACACAATGAGTCCCGCCAGCTCCAAACGGCATGTATTCCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGC
   
Sequence Name 20-K118470-0022-047-B01C-8409
GenBank Accession LN495422 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K118470-0022-047-B01C-8409
CTGGAAACATGCCGTTTGGAGCTGGCGGGACTCATTGTGTAGGGTCAGAGTTTGCTAAGC
TTATAATTGCAATCTTGCTGCATCATTTGTCCAGATTCAGGTTAATTAATTTAAACTAAG
TGTTTATTAAACCAAATTGAAAAAATAGATTGTTACTTAAATGATGGGAATTATTTTTGT
CAGGTGGTCATTGGATCCAAAAACAGAAGTACTTCGAAGGTATACACTTGTGTTCCCGGC
TGGATGTGTAGTCCACATCTCCAAGGAATCGGAGTGATGATCATTGAATTTTGGTTTGGC
ATTACATTTCTTGTACAATAATTAACGATTATTGACTTTGTTCCTGGCAT

Last Updated on 06.12.2018 16:11