Sequences from confirmation amplicon


Confirmation sequences for line 048A08 and hit At4g29035 are shown.
Line ID 048A08
Locus At4g29035 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14310633
Primers used for PCR TA14 - 8474, show primer details
   
Sequence Name 08-K030138-0022-048-A08C-TA14
GenBank Accession LN489610 [GenBank]
Sequencing primer gene specific primer, TA14: 5'-ACCAACCTTTCAGCTTCAATTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K030138-0022-048-A08C-TA14
TGATTTGCAGCATGACTCTTCATTAATGCGATGTAATAATATCAAAATTACCGTTTCACT
TTCCCACTATAAAAAGAAGTAACAATAAAAATTCTTCCTAAAGTTTTTGCTTGCCCGGAA
AAAAAATGAATTACATCAAACAATTTATCTTGGCAATTTGCTTTTATCTAGTCTTAACAT
GTCAAGATCATGTTTTAGCCCGCGACACCACCACGAGAGACATCGTAGTCCCGAAGATTT
CCGAGTGGCAAGTTACGGTTGCTAACGGTTTGACCACTGGTGAAACTCTTTTCATCCATT
GTAAGTCTAAGGAAAACGATCTAGGTGATATTAACCTGAAGTTCTTAGATAGATTTTCTT
GGAATTTTGGAGAAAATATGCTTCACTCAACTTTGTTTTGGTGCTATATGAGCAAAGATG
ATGGTCATATGAATGTAAAAGTGTGAGTATGAATGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTT
TGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATT
CTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 08-K030138-0022-048-A08C-8409
GenBank Accession LN489609 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K030138-0022-048-A08C-8409
ATTTCATTCATACTCACACTTTTACATTCATATGACCATCATCTTTGCTCATATAGCACC
AAAACAAAGTTGAGTGAAGCATATTTTCTCCAAAATTCCAAGAAAATCTATCTAAGAACT
TCAGGTTAATATCACCTAGATCGTTTTCCTTAGACTTACAATGGATGAAAAGAGTTTCAC
CAGTGGTCAAACCGTTAGCAACCGTAACTTGCCACTCGGAAATCTTCGGGACTACGATGT
CTCTCGTGGTGGTGTCGCGGGCTAAAACATGATCTTGACATGTTAAGACTAGATAAAAGC
AAATTGCCAAGATAAATTGTTTGATGTAATTCATTTTTTTTCCGGGCAAGCAAAAACTTT
AGGAAGAATTTTTATTGTTACTTCTTTTTATAGTGGGAAAGTGAAACGGTAATTTTGATA
TTATTACATCGCATTAATGAAGAGTCATGCTGCAAATCATTTACGTGATCATAGGTTAAT
TAGAATTGAAGCTGAAAGGTTGG

Last Updated on 06.12.2018 16:11