Sequences from confirmation amplicon


Confirmation sequences for line 048G02 and hit At1g59760 are shown.
Line ID 048G02
Locus At1g59760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21985718
Primers used for PCR NC02 - 8474, show primer details
   
Sequence Name 03-K101267-0022-048-G02C-8409
GenBank Accession LN487413 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K101267-0022-048-G02C-8409
TTCGGACATGAGCCTTTACATTGAATACCACCTTCCTCAACTTCAGGATCCTGAAGTTGA
TATGAAGGTATTTTGTTTTTCCCCCAGTACCTTTCTGCTTCTGCTCTTTCATTTGTCACA
AGAGATGAATACAACTTTTACTTCATTAGATTAAGAGCAGCTCTTACAAAAAGACAGTTC
GGCGGCTGGAGGCTCTGGAAAACCTATTTGAAAAACACAAAATTGCAAAATCTCCACTCA
TAACAGAAAAGTTGAAAGTTCTACAGATGAAGGAAGAGCTAATAGCCAAGATCAAGTCAC
TTAAGAAAACTGTCCGATCGTCAACAGCATTGGCGTTTAAAGATGAACTCAAGGCCAGAA
AGCGTGTTTTACGAAGGCTAGGGTAAATATTTGCTAAATTTATTTCATTCACTTTCAAAA
TATACTTGTGAAACCAACTCAATCTTATACGTCAGCTACATCATTCTATCATGCTCTTTT
TAATGTTATGGGACTGTATGTTCCATTGCTGATTCTATTAGCTTAATTTTTATCATTATG
CTATTGAACGCAGATACATCACTAGTGATAATGTCGTGGAGTTGAAGGGTAAGGTTGCAT
GTGAAATCAGTAGTGCAGAAGAATTGACATTAACGGAGTTAATGTTCAGTGGTATCTTCA
AGGATGCAAAGGTGGAGGAGTTGGTTTCGCTCCTCTCTTGCTTTGTATGGCGAGAGAGAC
TCCCCGACGCTGCTNAACCCAGAGAAGAACTCGACTTGCTCTTCATACAGTTACAAGACA
CAGCCCGGCGTGTAGCTAAAGTTCAGCTTGACTGCAAGGTATTCACTAACATATTCTCTC
TGCTCA
   
Sequence Name 03-K101273-0022-048-G02C-NC02
GenBank Accession LN487414 [GenBank]
Sequencing primer gene specific primer, NC02: 5'-TTCACTTTTAGCTGAGCAGAGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K101273-0022-048-G02C-NC02
AGCTGACTTCGCTACCGCCTGGCTGTGTCTTGTAACTGTATGAAGAGCAAGTCGAGTTCT
TCTCTGGGTTTAGCAGCGTCGGGGAGTCTCTCTCGCCATACAAAGCAAGAGAGGAGCGAA
ACCAACTCCTCCACCTTTGCATCCTTGAAGATACCACTGAACATTAACTCCGTTAATGTC
AATTCTTCTGCACTACTGATTTCACATGCAACCTTACCCTTCAACTCCACGACATTATCA
CTAGTGATGTATCTGCGTTCAATAGCATAATGATAAAAATTAAGCTAATAGAATCAGCAA
TGGAACATACAGTCCCATAACATTAAAAAGAGCATGATAGAATGATGTAGCTGACGTATA
AGATTGAGTTGGTTTCACAAGTATATTTTGAAAGTGAATGAAATAAATTTAGCAAATATT
TACCCTAGCCTTCGTAAAACACGCTTTCTGGCCTTGAGTTCATCTTTAAACGCCAATGCT
GTTGACGATCGGACAGTTTTCTTAAGTGACTTGATCTTGGCTATTAGCTCTTCCTTCATC
TGTAGAACTTTCAACTTTTCTGTTATGAGTGGAGATTTTGCAATTTTGTGTTTTTCAAAT
AGGTTTTCCAGAGCCTCCAGCCGCCGAACTGTCTTTTTGTAAGAGCTGCTCTTAATCTAA
TGAAGTAAAAGTTGTATTCATCTCTGGTGACAAATGAAAGAGCAGAAGCAGAAAGGTACT
GGGGGAAAAACAAAATACCTTCATATCAACTTCAGGATCCTGAAGTTGAGGAAGGTGGTA
TTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTCAATTCAAAATGT

Last Updated on 06.12.2018 16:11