Sequences from confirmation amplicon


Confirmation sequences for line 049B11 and hit At3g17680 are shown.
Line ID 049B11
Locus At3g17680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6043801
Primers used for PCR PA20 - 8474, show primer details
   
Sequence Name 21-K037017-022-049-B11C-8409
GenBank Accession LN495576 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K037017-022-049-B11C-8409
CATTTACAATTGACTGCATTTCGGACATTGATTCCGTTAGTGAGGTCAGTTCTGAAGTTG
AGAGCACCTTATCCTTTCAACAGATGAAAGATCCTACTGCGGTTTCTGAGAAAGTCGATG
AGCTTGTATCCCAACTTGTGACAGCAAGCTTGGACAAAGAGATTCTGAAACACGAGTTAC
TTCACAAGGACCAACAGTTTCATGAAGCCTCCAAGACTATAGAGCTGTTGAAGAAGTTTG
TCATGTTGCTGGAGATGGAGAAAGAAGTGGCTGTGGAGGAAAACGCTAATCTTGGCTACA
AACTCACTTCTCTCTTGGAGGAGAACAGAGAGCTAGCCACTGAGGCATTGTTCATGAAGA
ACGAAGCTGTTGGGCTTGCTAGGTGTGTGCTTAAGATGAGAGATGACCACTTTCACAAGG
TCTGCATTCTCCAGAACCGCATCTACTCGCTTCAGGCATCTAGGAACTCAGAGCCCGTCT
CTGATAAGTTCTCATACAA
   
Sequence Name 22-K037017-022-049-B11C-PA20
GenBank Accession LN496024 [GenBank]
Sequencing primer gene specific primer, PA20: 5'-GTATGAGACCTTATCAGAGACGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K037017-022-049-B11C-PA20
TGCGGTTCTGGAGAATGCAGACCTTGTGAAAGTGGTCATCTCTCATCTTAAGCACACACC
TAGCAAGCCCAACAGCTTCGTTCTTCATGAACAATGCCTCAGTGGCTAGCTCTCTGTTCT
CCTCCAAGAGAGAAGTGAGTTTGTAGCCAAGATTAGCGTTTTCCTCCACAGCCACTTCTT
TCTCCATCTCCAGCAACATGACAAACTTCTTCAACAGCTCTATAGTCTTGGAGGCTTCAT
GAAACTGTTGGTCCTTGTGAAGTAACTCGTGTTTCAGAATCTCTTTGTCCAAGCTTGCTG
TCACAAGTTGGGATACAAGCTCATCGACTTTCTCAGAAACCGCAGTAGGATCTTTCATCT
GTTGAAAGGATAAGGTGCTCTCAACTTCAGAACTGACCTCACTAACGGAATCAATGTCCG
AAATGCAGTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT
TCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11