Sequences from confirmation amplicon


Confirmation sequences for line 049C01 and hit At5g02070 are shown.
Line ID 049C01
Locus At5g02070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:406836
Primers used for PCR DL59 - 8474, show primer details
   
Sequence Name 14-K018513-022-049-C01C-DL59
GenBank Accession LN492297 [GenBank]
Sequencing primer gene specific primer, DL59: 5'-CTTGTTGGGTAAATCAAAATGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K018513-022-049-C01C-DL59
ACGAGTCTCGGCCTGAAACGATGCTCCTGCAAAAAAGGTTTGGAATGGGATCCGGTTAAC
GCAATATGTAAGTAAACTGCATAACAAGTGAACTATTCAAAACAAATTTCTTGAATCAGA
TCATTGTGATTGATTAGTTCTTGGCATACATGTATTAGGTGGTAAATGTCGCCATGGGAA
ACACTGCAAAAAGAAGAAGAAGACCGTTGTTTTCGCAGGTACAGAGGATGAACCGAAAAA
CCAAAAATATATTCAAATGCCCATATGATTGTACTCCATTTTAACTTCTTTGCTCTTGAA
GGTGCGGCTGTTGCGGTTGTGGGAGTTACGCTAGCGATTGCGGTAGCAGTAATTGGAACC
AAACATAGCCACCAAAAGGTTAAAAAGGATATCCACAAGAACATTGTTAAGGAGCGAGAA
GAGATGCTTAGCGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 15-K018513-022-049-C01C-8409
GenBank Accession LN492759 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K018513-022-049-C01C-8409
CATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCGCTAAGCATCTCTTCTC
GCTCCTTAACAATGTTCTTGTGGATATCCTTTTTAACCTTTTGGTGGCTATGTTTGGTTC
CAATTACTGCTACCGCAATCGCTAGCGTAACTCCCACAACCGCAACAGCCGCACCTTCAA
GAGCAAAGAAGTTAAAATGGAGTACAATCATATGGGCATTTGAATATATTTTTGGTTTTT
CGGTTCATCCTCTGTACCTGCGAAAACAACGGTCTTCTTCTTCTTTTTGCAGTGTTTCCC
ATGGCGACATTTACCACCTAATACATGTATGCCAAGAACTAATCAATCACAATGATCTGA
TTCAAGAAATTTGTTTTGAATAGTTCACTTGTTATGCAGTTTACTTACATATTGCGTTAA
CCGGATCCCATTCCAAACCTTTTTTGCAGGAGCATCGTTTCAGGCCGAGACTCGTTGGAT
CAGGCAAGCATTTTGATTT
Primers used for PCR FM04 - 3144, show primer details
   
Sequence Name 17-K029299-022-049-C01C-3144
GenBank Accession LN493724 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K029299-022-049-C01C-3144
ATCCCTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGCCAGAGGA
CAGGGTACCCGGGGATCAGATTGTCGTTTTATCAAAATGTACTTTCATTTTATAATAACG
CTGCGGACATCTACATTTTTGAATTGAAAAAAAATTGGTAATTACTCTTTCTTTTTCTCC
ATATTGACCATCATACTCATTGCTGATCCATGTAGATTTCCCGGACATGAAGCCATTTAC
AATTGAATATATCCCGGAAAATCATCAAGAATCTTCACTGGCAGAGAAATCACGAAAGCA
ACCAACAATTTCTCAAAAGACAATCTCATAGGCACTGGAGGTTTTGGAGAAGTGTTTAAA
GCTGTTCTTGAGGATGGAACCATAACCGCGATCAAACGAGCAAAGCTTAACAACACCAAA
GGCACTGATCAGATCCTAAACGAAGTCCGGATTCTCTGCCAAGTCAATCATAGAAGCTTA
GTTAGGCTTCTCGGTTGCTGTGTTGATCTTGAATTGCCTCTCTTGATCTACGAGTTTATT
CCCAACGGAACGCTGTTCGAACATCTCCATGGCAGTTCAGACCGTACTTGGAAACCGCTG
ACTTGGCGGCGCAGACTGCAGATTGCATACCAAACCGCTGAAGGATTAGCTTATCTCCAC
TCCGCAGCACAGCCACCCATTTACCACCGCGACGTATAGTCAAGCAATATTTTGCTTGAC
GAGAAGCTAAACGCCAAGGTTTCAGACTTTGGGCTGTCAAGACTTGT
   
Sequence Name 18-K029299-022-049-C01C-FM04
GenBank Accession LN494173 [GenBank]
Sequencing primer gene specific primer, FM04: 5'-CTCTCGTTATTAGCGGTTTCTGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K029299-022-049-C01C-FM04
CAAGTCTGAACCTTGGCGTTAGCTTCTCGTCAAGCAAAATATTGCTTGACCTTACGTCGC
GGTGGTAAATGGGTGGCTGTGCTGCGGAGTGGAGATAAGCTAATCCTTCAGCGGTTTGGT
ATGCAATCTGCAGTCTGCGCCGCCAAGTCAGCGGTTTCCAAGTACGGTCTGAACTGCCAT
GGAGATGTTCGAACAGCGTTCCGTTGGGAATAAACTCGTAGATCAAGAGAGGCAATTCAA
GATCAACACAGCAACCGAGAAGCCTAACTAAGCTTCTATGATTGACTTGGCAGAGAATCC
GGACTTCGTTTAGGATCTGATCAGTGCCTTTGGTGTTGTTAAGCTTTGCTCGTTTGATCG
CGGTTATGGTTCCATCCTCAAGAACAGCTTTAAACACTTCTCCAAAACCTCCAGTGCCTA
TGAGATTGTCTTTTGAGAAATTGTTGGTTGCTTTCGTGATTTCTCTGCCAGTGAAGATTC
TTGATGATTTTCCGGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATA
GAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATA
TCACATCAATCCACTTGCTTT

Last Updated on 06.12.2018 16:11