Sequences from confirmation amplicon


Confirmation sequences for line 049C10 and hit At3g21890 are shown.
Line ID 049C10
Locus At3g21890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7709068
Primers used for PCR IL23 - 8474, show primer details
   
Sequence Name 16-K032413-0022-049-C10C-8409
GenBank Accession LN493327 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K032413-0022-049-C10C-8409
GAGCCTTTGCTAGTAGAAAACTCAATAACAACAAACGATCCTTGTTTCTTCCCTTTCTAT
TGCAGATATTTTTTGCTATTTTTAGTCACTACCCATTTCTTAAAATTCTTTTTTTTTTTC
CATTACACATTTCTAAAAATTCTAAGCTTCAAAAAATTAAAATTTTCAAATTTTTCTTAA
AAAAAAATTGCATAAAACCCCTAAAATATTTATAGCTTACCCCAAAAAAATCTAATAATC
AAAAAAAAACAAACGGAACCCCAGGATTATCTGGTTCACTTCTATTCCCCACGGGGGTTT
CCCCAACCGTCGTCCCCGACGGTAAAACAACGGGG
   
Sequence Name 16-K032413-0022-049-C10C-IL23
GenBank Accession LN493328 [GenBank]
Sequencing primer gene specific primer, IL23: 5'-GATCATGACTTCCACGTTGTTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K032413-0022-049-C10C-IL23
TAGAAGTGAACAAGATAATCATGAGGTTCCGTTTGTTTTTCTCTGATTATTAGATTTTTT
TGGTGTAAGCTATAAATATTCTAGGGTTTTTATGCAATTTTCTTTTAAGAAAAATCTGAA
AATTTTAATTTTATGAAGCTTAGAATTTTAAGAAATGTGTAATGAAAAAAAAAAAAAAAT
TTTAAAAAAGGGGTAGGGACTAAAAATAGCAAAAAATATCTGCAATAAAAGGGGAAAAAA
CAGGGATCGTTTGTTGTTATTGAGTTATTCTACAAAGCAAAGGGCTTCATGTCCGGGAAA
TCTACATGGATCACCAATGAGTATGAGGGTCAATATGGAAAAAAAAAAAGAGTAATTACC
ATTTTTTTTTCAATTCAAAAATGTAAATGTCCCCACCGTTTTTATAAAATGAAAGTACTT
TTTGATAAAACGACAAATTACAATCCGTCGTATTTATGGGCAAAAGCAATAAACAAATTA
TTCTAATTCGAAAATCTTTATTTCAACGTGTCTACTTTCC

Last Updated on 06.12.2018 16:11