Sequences from confirmation amplicon


Confirmation sequences for line 050A04 and hit At1g68170 are shown.
Line ID 050A04
Locus At1g68170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25553300
Primers used for PCR 06cp - 8474, show primer details
   
Sequence Name 59-K108745-0022-050-A04C-8409
GenBank Accession LN510022 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K108745-0022-050-A04C-8409
GCCATTTACATTGCATGTAGAGTGTGTGAGTTGACCATATTCCTCTATTCCTCTATAGAA
TATAAAGACAAGAGCTCCACCAACACCAAACAATGTTCCAAAAACCTTAGCCAGCCCTAC
GCTCGATCCAAGCCGTACACTCTCCATCCTATGAGACCCATAAAAGTTTATTATATTTTG
TTTAATGGTATAAACATATATTAAAAGGGGTTAACTAAATTGTGATTCGTTGATTAGACT
TGCCTAAGAAGTGCAGCGAAGATGAAAGTGACCAATGGAGTGAGAACGCCAGCAGCAGAG
GTGAATGTTGCTGACGTTAGAGCTAGACCCGTAATGGTTAGGATACTTGGTATCACCACA
CTGTTCAATTATCACCA
   
Sequence Name 75-K108746-0022-050-A04C-06cp
GenBank Accession LN513257 [GenBank]
Sequencing primer gene specific primer, 06cp: 5'-GTGGTGATAATTGAACAGTGTGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K108746-0022-050-A04C-06cp
CAACATGTCACCTCTGCTGCTGGCGTTCTCACTCCACTGGTCACTTTCATCTTCGCTGCA
CTTCTTAGGCAAGTCTAATCAACGAATCACAATTTAGTTAACCCCTTTTAATATATGTTT
ATACCATTAAACAAAATATAATAAACTTTTATGGGTCTCATAGGATGGAGAGTGTACGGC
TTGGATCGAGCGTAGGGCTGGCTAAGGTTTTTGGAACATTGTTTGGTGTTGGTGGAGCTC
TTGTCTTTATATTCTATAGAGGAATAGAGGAATATGGTCAACTCACACACTCTACATGCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTA

Last Updated on 06.12.2018 16:11