Sequences from confirmation amplicon


Confirmation sequences for line 050B09 and hit At2g15640 are shown.
Line ID 050B09
Locus At2g15640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:6817295
Primers used for PCR CW52 - 8474, show primer details
   
Sequence Name 69-K104127-0022-050-B09C-CW52
GenBank Accession LN512194 [GenBank]
Sequencing primer gene specific primer, CW52: 5'-TTCAGCCTTTCACTTAACTCCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K104127-0022-050-B09C-CW52
CTCGTTGCCCCTCCTAGTCTGATTCTGAATCTGGCTCATCCACCAATGGGGGATGGACTG
ACTTGAGTTGTTTAAAGTCGTGAACATTAAGATCCTCTACGTGGTTTACAAAGGTGCAAA
CTCTACAAGTTTTCTTTAACACTTCACCAAAACCTTGGATTTCAACACGTTGGAGAGTGT
TCCTTTCGGGATTGAAGTAGAAAACATAAAACGGTTGCTTAGGTGTATATTTGAGCATCG
AAAACACTATTTCACCCGAAGCAGTCCCTCCAGCGATGGAAACACGACGACGGAAGAATC
TATCATCGGTCCAAGTGTAGGCATATTTCGACCATTGCTGTTTCTTAACATCCTCTAGAA
CCCACACACGCAACTCATTAATGTCATCATCGTTAATATTATACTCCACATATTCGTCGA
CATCCGAATAATAAAGCTTATAAATGTCGACATCATCCTCCCAATAAATCACAGCTAATT
TTCCCTTATAGTTGATCAATCGACAAAACCTTTCTACATCAGGATATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 82-K104128-0022-050-B09C-8409
GenBank Accession LN514187 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K104128-0022-050-B09C-8409
TGAAGCCACTTACAATTGAATATATCCTGATGTAGAAAGGTTTTGTCGATTGATCAACTA
TAAGGGAAAATTAGCTGTGATTTATTGGGAGGATGATGTCGACATTTATAAGCTTTATTA
TTCGGATGTCGACGAATATGTGGAGTATAATATTAACGATGATGACATTAATGAGTTGCG
TGTGTGGGTTCTAGAGGATGTTAAGAAACAGCAATGGTCGAAATATGCCTACACTTGGAC
CGATGATAGATTCTTCCGTCGTCGTGTTTCCATCGCTGGAGGGACTGCTTCGGGTGAAAT
AGTGTTTTCGATGCTCAAATATACACCTAAGCAACCGTTTTATGTTTTCTACTTCAATCC
CGAAAGGAACACTCTCCAACGTGTTGAAATCCAAGGTTTTGGTGAAGTGTTAAAGAAAAC
TTGTAGAGTTTGCACCTTTGTAAACCACGTAGAGGATCTTAATGTTCACGACTTTAAACA
ACTCAAGTCAGTCCATCCCCCATTGGTGGATGAGCCAGATTCAGAATCAGACTAGGAGGG
GCAACGAGAAGAAGGACATAGACGATCATTGGAGTTAAGTGAAAAGGCTGA

Last Updated on 06.12.2018 16:11