Sequences from confirmation amplicon


Confirmation sequences for line 050C01 and hit At1g10450 are shown.
Line ID 050C01
Locus At1g10450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3434625
Primers used for PCR 07uv - 3144, show primer details
   
Sequence Name 66-K117671-0022-050-C01C-07uv
GenBank Accession LN511695 [GenBank]
Sequencing primer gene specific primer, 07uv: 5'-AGGAAGGACAATAGCCACACTAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K117671-0022-050-C01C-07uv
TTTTCATACATATAGTAGAGTCTACTGAGATAGTGTTGTTGTTCATCTTTTCGAGAAGGA
TTTCCACCTGTTTAATGGCTGAACTTACAGATCCTAGTAACATGTCCATTTCATACCTGA
ACACAAATTACAGAAATGTTAAACTGGGGGATTCTACCAATAAAAGGAAAACTGAAGGCG
GGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTAT
ATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTACCTGAACACTGATAGTTTAAACC
GAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACT
TCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTC
AGTGGAGATATCACATCAATCCAC
   
Sequence Name 18-K117670-0022-050-C01C-3144
GenBank Accession LN494489 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K117670-0022-050-C01C-3144
CCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTCAGGTACAATCCC
ACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAG
GGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCAGTTTTCCTTTTATTGGTAGAATCC
CCCAGTTTAACATTTCTGTAATTTGTGTTCAGGTATGAAATGGACATGTTACTAGGATCT
GTAAGTTCAGCCATTAAACAAGTGGAAATCCTTCTCGAAAAGATGAACAACAACACTATC
TCAGTAGACTCTACTATATGTATCGAAAAACACTTATCAGGTAAGTTTTTAGTTTGGAAC
AAATCTAGTGTGGCT

Last Updated on 06.12.2018 16:11