Sequences from confirmation amplicon


Confirmation sequences for line 050C04 and hit At1g26150 are shown.
Line ID 050C04
Locus At1g26150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9042620
Primers used for PCR RK60 - 3144, show primer details
   
Sequence Name 39-K018968-022-050-C04C-RK60
GenBank Accession LN503192 [GenBank]
Sequencing primer gene specific primer, RK60: 5'-GAAGCCTGTTGTCCGAGTATTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K018968-022-050-C04C-RK60
AAATTATCTACAGTGTTGAGTGATGTGTGGTTTTTAGTTGGTGCTTGGAGGTTACTGTCA
TACTCAAGTCTGGGAATTTTTGTTTCTTTTTTGTTTAATAATTTCTCTTAGTAAAGTAAG
TGACGGTGTTTGTTCAATTTCAGTTTTTGATCAGAAGAGTGTTGGTGAAGAAGATGTTAG
AATCTGGTCTTGGGATTTAGATGACTACACCTGCACAGGCTCCTAGGGAGGAGGTGTCAC
TGTCTCCATCATTGGCGTCCCCTCCACTAATGGCGTTGCCTCCACCGCAGCCTTCGTTTC
CCGGTGATAATGCCACTTCCCCAACTAGAGAGCCCACTAATGGCAATCCACCAGAGACCA
CAAACACCCCAGCTCAGTCTTCTCCTCCTCCTGAGACTCCACTATCATCACCACTTGTAC
ACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCT
CCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTG
CGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTG
GAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGG
   
Sequence Name 40-K018968-022-050-C04C-3144
GenBank Accession LN503550 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K018968-022-050-C04C-3144
AGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCAT
TTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAA
ACTATCAGTGTACAAGTGGTGATGATAGTGGAGTCTCAGGAGGAGGAGAAGACTGAGCTG
GGGTGTTTGTGGTCTCTGGTGGATTGCCATTAGTGGGCTCTCTAGTTGGGGAAGTGGCAT
TATCACCGGGAAACGAAGGCTGCGGTGGAGGCAACGCCATTAGTGGAGGGGACGCCAATG
ATGGAGACAGTGACACCTCCTCCCTAGGAGCCTGTGCAGGTGTAGTCATCTAAATCCCAA
GACCAGATTCTAACATCTTCTTCACCAACACTCTTCTGATCAAAAACTGAAATTGAACAA
ACACCGTCACTTACTTTACTAAGAGAAATTATTAAACAAAAAAGAAACAAAAATTCCCAG
ACTTGAGTATGACAGTAACCTCCAAGCACCAACTAAAAACCACACATCACTCAACACTGT
ACTATAATTTGAACCCTAAATACTCGGA
Primers used for PCR FI12 - 8474, show primer details
   
Sequence Name 19-K028096-022-050-C04C-8409
GenBank Accession LN494619 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K028096-022-050-C04C-8409
AGCCATTTACATTGAGCTCCTCCGACGACGATTCCAGTATCTCCTCCACCAGAACCTTCT
CCCCCACCACCTTTACCAACTGAAGCTCCTCCTCCAGCGAATCCAGTATCATCACCTCCA
CCAGAATCTTCTCCCCCGCCACCTCCACCAACTGAAGCTCCTCCTACTACTCCTATTACA
TCACCCAGCCCGCCAACAAATCCACCACCACCACCGGAAAGCCCTCCTTCACTACCTGCA
CCAGACCCTCCAAGCAATCCACTTCCACCACCTAAGTTAGTCCCACCATCACATTCACCT
CCACGTCATTTACCTTCCCCGCCAGCCTCAGAAATTCCTCCTCCTCCACGTCATTTACCT
TCCCCACCAGCCTCAGAACGTCCTTCAACTCCTCCATCAGATTCAGAACATCCTTCTCCT
CCTCCTCCAGGGCATCCAAAACGTCGTGAGCAACCTCCTCCACCGGGTTCTAAACGTCCT
ACTCNATCAC
   
Sequence Name 19-K028096-022-050-C04C-FI12
GenBank Accession LN494620 [GenBank]
Sequencing primer gene specific primer, FI12: 5'-GAGGTGATGGAGTAGGACGTTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K028096-022-050-C04C-FI12
TTTTGGATGCCCTGGAGGAGGAGGAGAAGGATGTTCTGAATCTGATGGAGGAGTTGAAGG
ACGTTCTGAGGCTGGTGGGGAAGGTAAATGACGTGGAGGAGGAGGAATTTCTGAGGCTGG
CGGGGAAGGTAAATGACGTGGAGGTGAATGTGATGGTGGGACTAACTTAGGTGGTGGAAG
TGGATTGCTTGGAGGGTCTGGTGCAGGTAGTGAAGGAGGGCTTTCCGGTGGTGGTGGTGG
ATTTGTTGGCGGGCTGGGTGATGTAATAGGAGTAGTAGGAGGAGCTTCATTTGGTGGAGG
TGGCGGGGGAGAACATTCTGGTGGGGGT

Last Updated on 06.12.2018 16:11