Sequences from confirmation amplicon


Confirmation sequences for line 050D12 and hit At4g36910 are shown.
Line ID 050D12
Locus At4g36910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17392061
Primers used for PCR IN09 - 3144, show primer details
   
Sequence Name 34-K033542-022-050-D12C-IN09
GenBank Accession LN501316 [GenBank]
Sequencing primer gene specific primer, IN09: 5'-AGTTCATCAGATTCTTTGGAGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K033542-022-050-D12C-IN09
GCCTCTTTTGTGCCTTCTCTACAGCTCTGGAACTCCTTGTGGAGAATAGAATCACTGGAT
TTCCTGTAATTGACGAAGACTGGAAATTGGTTAGTATCAACTTTTGTTATTCAGGTCTTA
AGTTTTGAAAGGGAGCTGAATCTAATCTACAGATGGAGGTTGGATGCTTATGAGGGATTC
TTTGTTGCTGCAGGTTGGGCTTGTTTCAGATTATGACTTGTTGGCTTTGGACTCCATATC
TGGTAAGTGGGCCAGCTACACCACTTAATTAATGTATTTGGTTTCCTTTGCTTTCAAGAG
ATTTTATAAACATGGGGATTTCTCTTTGAAGTGGAGAAAGAAGAAAGCTACTTGATCACA
TGGTGGCATAAAGCCAGAAATATCTATGTTATGATGACCTTCAAACACTGATAGTTTAAA
CCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAA
CTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACG
TCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTT
TCCACGATGCTCCTCGTGGGTGGG
   
Sequence Name 34-K033542-022-050-D12C-3144
GenBank Accession LN501315 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K033542-022-050-D12C-3144
TCCACATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAAG
GTCATCATAACATAGATATTTCTGGCTTTATGCCACCATGTGATCAAGTAGCTTTCTTCT
TTCTCCACTTCAAAGAGAAATCCCCATGTTTATAAAATCTCTTGAAAGCAAAGGAAACCA
AATACATTAATTAAGTGGTGTAGCTGGCCCACTTACCAGATATGGAGTCCAAAGCCAACA
AGTCATAATCTGAAACAAGCCCAACCTGCAGCAACAAAGAATCCCTCATAAGCATCCAAC
CTCCATCTGTAGATTAGATTCAGCTCCCTTTCAAAACTTAAGACCTGAATAACAAAAGTT
GATACTAACCAATTTCCAGTCTTCGTCAATTACAGGAAATCCAGTGATTCTATTCTCCAC
AAGGAGTTCCAGAGCTGTAGAGAAGGCACAAAAGAGGCCATAAGTATGAAAACAAGGCAG
TATCTTATGCTCCAAAG
Primers used for PCR IN08 - 8474, show primer details
   
Sequence Name 33-K033542-022-050-D12C-8409
GenBank Accession LN500896 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K033542-022-050-D12C-8409
ATTCTTTGCGAGATAGCACGCTCACCGTGAAATGATCAAAAAGCTTTTTCCCTTACCCCT
TGAATCTTATTGCTGGTTTTTTTGTGTTGCTAATAGGTAGTGGAAGAACAGAAAATTCCA
TGTTCCCTGAGGTTGACAGCACCTGGAAAGTATGTGGCCCTTCCTTTTCCTATTAACATT
TTGACCTGAATGCTTTTCAAAACACTCAATTCAAATTCTTTCCCATTGTGCTCATTCGCC
TGGTTCACATTATCAGCAGTAGATAGTGTGTATTGAAAGTTACAAGGAACTTGTTTTGAT
TTACGCAATTACCATGCACAATAAT
   
Sequence Name 33-K033542-022-050-D12C-IN08
GenBank Accession LN500897 [GenBank]
Sequencing primer gene specific primer, IN08: 5'-ATTATTGTGCATGGTAATTGCGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K033542-022-050-D12C-IN08
CTTTCATACACACTATCTACTGCTGATAATGTGAACCAGGCGAATGAGCACAATGGGAAA
GAATTTGAATTGAGTGTTTTGAAAAGCATTCAGGTCAAAATGTTAATAGGAAAAGGAAGG
GCCACATACTTTCCAGGTGCTGTCAACCTCAGGGAACATGGAATTTTCTGTTCTTCCACT
ACCTATTAGCAACACAAAAAAACCAGCAATAAGATTCAAGGGGTAAGGGAAAAAGCTTTT
TGATCATTTCACGGTGAGCGTGCTATCTCGCAAAGAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAAATGG

Last Updated on 06.12.2018 16:11