Sequences from confirmation amplicon


Confirmation sequences for line 050E10 and hit At3g51190 are shown.
Line ID 050E10
Locus At3g51190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19017536
Primers used for PCR CW50 - 8474, show primer details
   
Sequence Name 70-K104127-0022-050-E10C-CW50
GenBank Accession LN512380 [GenBank]
Sequencing primer gene specific primer, CW50: 5'-TATTCATGCTTATATTGCAGGCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K104127-0022-050-E10C-CW50
TTTATTATAATGGCTTTGATAGTCCGGTACTTCTATACCGGTGATAAACACAAAGCAAAA
GTATAGCCCTCTAAACTGTACATACAATCGAAATATATTTTTTGATTTCTTAAATTGGTT
GTATAGAATTGGAATAACAATGTAGTTCAACAATTTTGCATCGGCTGTCACTTGTCAGCA
AAATATATAATTTGTAACGTAACGTATAAAGAGGTCCACTTTCTAAGATATATGAGATTT
TGACAGCATACAATTTACGTTTCTATTAAAGAATCTGTCTCTTTTTATTTTAGTTGTTAT
ACATACCCGTAATTAAAATGCAAATATAAGTATAGAATAATCTGAATTTTTGACACAATA
ATAAAAAGTAAAACTTTTACATAACATTGGGTTAACATAACTGGGCCAGTATTTCTAACA
TAACTGGGCCTGCAGTCTATTTACAAAGAAGGTTACATGAAGGCCCCATAAGCCGACGAC
TATNAATTAGGTAGGGTTTCTGTTCTTTGACAAAACATTTTAGGGTTTTTGTGCTTATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCG
   
Sequence Name 83-K104128-0022-050-E10C-8409
GenBank Accession LN514307 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K104128-0022-050-E10C-8409
TTACAATTGAATATAAGCACAAAAACCCTAAAATGTTTTGTCAAAGAACAGAAACCCTAC
CTAATTCATAGTCGTCGGCTTATGGGGCCTTCATGTAACCTTCTTTGTAAATAGACTGCA
GGCCCAGTTATGTTAGAAATACTGGCCCAGTTATGTTAACCCAATGTTATGTAAAAGTTT
TACTTTTTATTATTGTGTCAAAAATTCAGATTATTCTATACTTATATTTGCATTTTAATT
ACGGGTATGTATAACAACTAAAATAAAAAGAGACAGATTCTTTAATAGAAACGTAAATTG
TATGCTGTCAAAATCTCATATATCTTAGAAAGTGGACCTCTTTATACGTTACGTTACAAA
TTATATATTTTGCTGACAAGTGACAGCCGATGCAAAATTGTTGAACTACATTGTTATTCC
AATTCTATACAACCAATTTAAGAAATCAAAAAATATATTTCGATTGTATGTACAGTTTAG
AGGGCTATACTTTTGCTTTGTGTTTATCACCGGTATAGAAGTACCGGACTATCAAAGCCA
TTATAATAAATGATCAACTTGCATGCTGCTGCCTGCAAT
Primers used for PCR CW51 - 3144, show primer details
   
Sequence Name 69-K104197-0022-050-E10C-CW51
GenBank Accession LN512196 [GenBank]
Sequencing primer gene specific primer, CW51: 5'-AATAATTGGTTTCCCGAAATTACC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K104197-0022-050-E10C-CW51
GTGAGCATTACGATGGCGTAATCCCAGAAGCCCTAGCGAGAGCACCACGGTCACCGACAT
GGAGCTCAACGTTACAGATAACAGCTCCCTCAGGGATTGATCCAAGGGGAAGAACGTTAC
CGACCATAAGATTAGCCTTCTTACCACAGTAGAGGTATTGTCCGGTGTACATACCTTCGG
CGGCTACGAACAGTTCCTTCTGCTTCATGTAGCGGAAAGGATGACGGAACGCGACACGAG
CGAGAGGAGCACCACGGCCTGGGTCGTGGATGATTTCGGTCACCAATCCTTTGAGGTAAC
CGTTTCGCTCTCCGTAATCGAGGCTCCGGAACTTAGCTGGACCCTTGCGGTGGTGGGTGT
GGGACATGAAAACGGAACCAGCCGCACCTTTACGCTGTGCCCTAATCTACATGATATATC
CCGGATCATGAAGCCATTTCATGTAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTC
CAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGC
GTCATCCCTTACGTCAGTGGAGATATC
   
Sequence Name 79-K104198-0022-050-E10C-3144
GenBank Accession LN513822 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K104198-0022-050-E10C-3144
TCCACTATCCTTCGCAAGACCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTACATGAAATGGCTTCATGATCCGGGATATATCA
TGTAGATTAGGGCACAGCGTAAAGGTGCGGCTGGTTCCGTTTTCATGTCCCACACCCACC
ACCGCAAGGGTCCAGCTAAGTTCCGGAGCCTCGATTACGGAGAGCGAAACGGTTACCTCA
AAGGATTGGTGACCGAAATCATCCACGACCCAGGCCGTGGTGCTCCTCTCGCTCGTGTCG
CGTTCCGTCATCCTTTCCGCTACATGAAGCAGAAGGAACTGTTCGTAGCCGCCGAAGGTA
TGTACACCGGACAATACCTCTACTGTGGTAAGAAGGCTAATCTTATGGTCGGTAACGTTC
TTCCCCTTGGATCAATCCCTGAGGGAGCTGTTATCTGTAACGTTGAGCTCCATGTCGGTG
ACCGTGGTGCTCTCGCTAGGGCTTCTGGTGATTACGCCATCGTAATTGCTCACAATCCTG
AAAGCAACACCACTAGGTAATTTCGGGAACCCAATTA

Last Updated on 06.12.2018 16:11