Sequences from confirmation amplicon


Confirmation sequences for line 051A08 and hit At3g43670 are shown.
Line ID 051A08
Locus At3g43670 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:15568145
Primers used for PCR 45yo - 8474, show primer details
   
Sequence Name 33-K121193-0022-051-A08C-45yo
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 45yo: 5'-ATCAATGTAGTAAGCGTTTCGAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K121193-0022-051-A08C-45yo
GGTCCTAACCAACTCTCCGCATCCATGTAAGCTTTTGAATACCAACCTTCCCCTGGATCC
ATATTCGGTACGAACAACTCCGACGCAAAACCTTTGTACATTACACTTCTTGCTTCACCT
GTTTCAACCTCAACAAATTGGGTTTCATTGTAACTTGGAAGACTTTTGGAGTCAATTTCT
AACAATAACTAAATTCAAGGGACTTGTTTGTGATATAATTAATTTACCTGTCTTGGAAAT
GGCTTCAATTGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 11-K121192-0022-051-A08C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K121192-0022-051-A08C-8409
TCGGAATTGAGCCTTTCCAGACAGGTAATTAATTATATCACAAACAAGTCCCTTGAATTT
AGTTATTGTTAGAAATTGACTCCAAAAGTCTTCCAAGTTACAATGAAACCCAATTTGTTG
AGGTTGAAACAGGTGAAGCAAGAAGTGTAATGTACAAAGGTTTTGCGTCGGAGTTGTTCG
TACCGAATATGGATCCAGGGGAAGGTTGGTATTCAAAAGCTTACATGGATGCTGGAGAGT
TTGGTTTAGGACCTTCTTCAATGCCACTTGTGCCACTCAACGACTGTCCTCGAAACGCTT
ACTACATTGA

Last Updated on 06.12.2018 16:11