Sequences from confirmation amplicon


Confirmation sequences for line 051A11 and hit At3g46350 are shown.
Line ID 051A11
Locus At3g46350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17036728
Primers used for PCR KE35 - 8474, show primer details
   
Sequence Name 28-K100995-0022-051-A11C-8409
GenBank Accession LN498763 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K100995-0022-051-A11C-8409
TTCGGACTGAGCCATTTACATTGAATATATCCTGCCGCCGCTGCCGCTTTGCACCCGGTG
GAGCTTGCATGTTGGTTTAGAGAAATTAATTGATCTTAGTAGATCTTAGTAGATGTTGAA
ATTAATTTTTTTGCGCTAATAGCTTTTTAAGTTTTAATTAAATACCTTTTTAACCTGTAT
AGGACAATTTTATCCGTTATTCATAAATGAATATTATTAGTATGCGAACATATTATATAA
TCAGTTGTGAATATTCAATATTGTATAGGACGATTGGTGAAGTCTGTTGTTGCATTCCTC
CCAGATCAAGTAGCCGATGTCTAATTTTAGGGTTGAGAGTCAAATAATATGCTATACTGC
TCAGCCAAAACATAATTTGTCTTGTTTGTTATCTTAAAGTTACTTTCATTTTTTTTTTTT
ACACTAAAATGTTGTAAAAAAATATAATATTAATATGACTATTTTCGATCGCCTAATCGC
ATAGGCCTAGTTTTAATACATTTTCTAATGTTCACATAAGTTTTATCTCTATATACATAA
TTATAAACGTTCATATATCGTTTGATATAAATTAAAAAAAACAGTTTCTTTAAAGTTTTG
TTTTTGATATAATATTTTTTTTTTCTAAAAAATATTTTCAAAAAATTTTTTAGGTATAAA
ATTATATTTCTAAGGTTTATGTTATATGCCTAAGGGCTAAAATTGCCATTAACCTTTTGC
CATATCTTAACAGGATT
   
Sequence Name 28-K100999-0022-051-A11C-KE35
GenBank Accession LN498764 [GenBank]
Sequencing primer gene specific primer, KE35: 5'-CCACAATCCAAACTTATGAATCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K100999-0022-051-A11C-KE35
GTTATGGCATATNAGCCATTAGGCATATAACATAAACATTAGAAATATAATATTATACAT
AAAAAATTTATAGAAAATATCTATTAGAAAAAAAAAATATTATATCAAAAACAAAACTTT
AAAGAAACTGTTTTTCTTAATTTATATCAAACGATATATGAACGTTTATAATTATGTATA
TAGAGATAAAACTTATGTGAACATTAGAAAATGTATTAAAACTAGGCCTATGCGATTAGG
CGATCGAAAATAGTCATATTAATATTATATTTTTTTACAACATTTTAGTGTAAAAAAAAA
AAATGAAAGTAACTTTAAAATAACAAACAAAACAAATTATGTTTTGGCTGAGCAGTATAG
CATATTATTTGACTCTCAACCCTAAAATTAAACATCGGCTACTTGATCTGGGAGGAATGC
AACAACAGACTTCCCCAATCGTCCTATACAATATTGAATATTCACAACTGATTATATAAT
ATGTTCGCATACTAATAATATTCATTTATGAATAACGGATAAAATTGTCCTATACAGGTT
AAAAAGGTATTTAATTAAAACTTAAAAAGCTATTAGCGCAAAAAAATTAATTTCAACATC
TACTAAGATCTACTAAGATCAATTAATTTCTCTAAACCAACATGCAAGCTCCACCGGGTG
CAAAGCGGCAGCGGCGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGT
Primers used for PCR KE36 - 3144, show primer details
   
Sequence Name 29-K100995-0022-051-A11C-3144
GenBank Accession LN499211 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K100995-0022-051-A11C-3144
TCCACTTCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGGTT
TACAATTGAATATAGTCGTCGATTGTTGGTTTCCTGCCGTTTCGAGGAAATTAATTTTTT
TCCTTTTCTCTAATTCATACAACCATTCCAACATCTACTAAGATCTTATCACCCTAGAAA
GTAGAAAGTAGAAACATTTCCAGTTTAACTACACCACTTCATGGAAGTTAATTTTATTAA
TTTCTTTTGCCGTAAGACAATAACAAAAACTAGAAATGCTAAGGTGCCAAACCTAACATC
AAAATGAGGATTACCTTCTTGTTCTTGAGCTTGAACAAGGTGAAAAATAGCAAAAGTTGC
TATAAGTGCAGTTAACAAAAGCTCGTGGGAACTGTTCATATTTTCTTATTTCCCAAACGA
AATAAAAACTGACCCAATTTCCTGAACCATAAACGAAGTTTATAAGCAATTAGGCCACAA
AACTCCTTTGGACTTCTCAAGTAAAACACCTTAACATGTCAATCCTCTGAAATCGTGAGG
A
   
Sequence Name 29-K100999-0022-051-A11C-KE36
GenBank Accession LN499212 [GenBank]
Sequencing primer gene specific primer, KE36: 5'-AGAAATTAGGACATCTCCTCACGA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K100999-0022-051-A11C-KE36
TAGGTGTTTACTTGAGAAGTCCAAAGGAGTTTTGTGGCCTAATTGCTTATAAACTTCGTT
TATGGTTCAGGAAATTGGGTCAGTTTTTATTTCGTTTGGGAAATAAGAAAATATGAACAG
TTCCCACGAGCTTTTGTTAACTGCACTTATAGCAACTTTTGCTATTTTTCACCTTGTTCA
AGCTCAAGAACAAGAAGGTAATCCTCATTTTGATGTTAGGTTTGGCACCTTAGCATTTCT
AGTTTTTGTTATTGTCTTACGGCAAAAGAAATTAATAAAATTAACTTCCATGAAGTGGTG
TAGTTAAACTGGAAATGTTTCTACTTTCTACTTTCTAGGGTGATAAGATCTTAGTAGATG
TTGGAATGGTTGTATGAATTAGAGAAAAGGAAAAAAATTAATTTCCTCGAAACGGCAGGA
AACCAACAATCGACGACTATATTCAATTGTAAACCAAACACTGATAGTTTAAACCGAAGG
CGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTT
ATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGG
AGAT

Last Updated on 06.12.2018 16:11