Sequences from confirmation amplicon


Confirmation sequences for line 051D01 and hit At2g29110 are shown.
Line ID 051D01
Locus At2g29110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12507546
Primers used for PCR AJ13 - 8474, show primer details
   
Sequence Name 07-K105456-0022-051-D01C-AJ13
GenBank Accession LN489267 [GenBank]
Sequencing primer gene specific primer, AJ13: 5'-AACATAGAGTCAACACCGACTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K105456-0022-051-D01C-AJ13
ATCGGCACTAGTTTTTGGTTCTCTTTCTCCACCATGGTTTTTGCCCACCGTAAGTTCTTA
CACCAATAATGATAAATGTTAACTTTCTTTTGAAAACTATATTATGTAAAATGAGACTAT
TGTTACATTGTTTATATATTAAATGTCAACTTATTTTCAATGTGATAATTTTTTTATTAA
CAAGAATCAAGATCCTAATGATGAAGTAATTTGTGTGTGTGCATATAGGTGAGAAGGTAG
TGAGCAACTTAGCAAGGTTTGTGGTGGTTGTATGGTGCTTTGTGGTGCTTGTGCTCACTC
AGAGCTACACAGCAAATCTCACCTCTTTCTTGACGGTGCAACGTTTTCAACCAGCGGCCA
TAAATGTGAAAGATCTCATAAAAAATGGGGACTATGTAGGCTATCAACACGGGGCTTTTG
TTAAAGATTTTCTAATAAAAGAGGGATTTAATGTTTCTAAGCTCAAGCCTTTTGGGTCTT
CCGAAGAATGTCACGCGCTTTTGTCCAATGGTAGTATATCAGCAGCTTTCGACGAAGTTG
CTTACCTTAGGGCTATCCTTTCTCAATACTGCTCCAAATATGCCATTGTTGAGCCTACAT
TTAAGACTGCTGGTTTTGGCTTTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCG
   
Sequence Name 23-K105457-0022-051-D01C-8409
GenBank Accession LN496553 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K105457-0022-051-D01C-8409
GCCATTTCAAGCCAAACCAGCAGTCTTAAATGTAGGCTCAACAATGGCATATTTGGAGCA
GTATTGAGAAAGGATAGCCCTAAGGTAAGCAACTTCGTCGAAAGCTGCTGATATACTACC
ATTGGACAAAAGCGCGTGACATTCTTCGGAAGACCCAAAAGGCTTGAGCTTAGAAACATT
AAATCCCTCTTTTATTAGAAAATCTTTAACAAAAGCCCCGTGTTGATAGCCTACATAGTC
CCCATTTTTTATGAGATCTTTCACATTTATGGCCGCTGGTTGAAAACGTTGCACCGTCAA
GAAAGAGGTGAGATTTGCTGTGTAGCTCTGAGTGAGCACAAGCACCACAAAGCACCATAC
AACCACCACAAACCTTGCTAAGTTGCTCACTACCTTCTCACCTATATGCACACACACAAA
TTACTTCATCATTAGGATCTTGATTCTTGTTAATAAAAAAATTATCACATTGAAAATAAG
TTGACATTTAATATATAAACAATGTAACAATAGTCTCATTTTACATAATATAGTTTTCAA
AAGAAAGTTAACATTTATCATTATTGGTGTAAGAACTTACGGTGGGCAAAAACCATGGTG
GAGAAAGAGAACCAAAAACTAGTGCCGATCTGGTGGTGAGGCGGTCCACGGAAGTCGGTG
TT
Primers used for PCR AL09 - 3144, show primer details
   
Sequence Name 83-K118835-0022-051-D01C-AL09
GenBank Accession LN514360 [GenBank]
Sequencing primer gene specific primer, AL09: 5'-ATATCGAATCTTCGGAATCATCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K118835-0022-051-D01C-AL09
AGAACCGAAGATGAGTAGTGCCAAGAATGAAGCAATGCCTGCTATTAGAAACAGACCCCA
AAAGCTACGAAGGCTTAGACGGTTCGACGAAAGAGCGGTCTTTGGATCAGGACAATCATT
TTGTTTCATGAACCATTTGTTCTCGATGTGTTGCATTTCATCTCCTTGAGTCACATTTAA
GATAGCCTTTGAGACATCTCCTGTCAAAGGTGAATTCCTGGGGAATGCCTACACATTGAT
CAAGGAAACAAGCAATGAATACTCGAACTGTCTTTTTTTTTTTTCCCAATTTGAAATTTT
CCATTTTTAGGGAAAAAAAAAAAAACCCCCCAAAAGGGTTTCCCCCACCCCCAAACCAAA
AGGGGAACCCCCAACCCCAAATTTCTTTTTTAAAGGTTCAAACCCCCTTGCCACAATTTT
TCCGGGGGGGGGCTCAAAAAAAATCCCCTCCCCGGAACAAGAAAAAACCGGTTGGAAACA
ACCCCCCTCCCTCCGGAATTGACCCTCAAACCCTTTGGGGAACCCGGGAAATTTCCCGGA
AATGAACCCTTTTACAATTAAAAATTTCCGGAATTTTTAAACCAAAGGGGGGAAAAAAAA
ATTTTATCCCCGGGTACCCTGTCCTCCCCAAAAAAAAAGAATTTCCTTAATATAAAAAAG
GGTTTTGCAAAAGAAAAGGGAATTTGGCCTCCCCCCTTCCCC
   
Sequence Name 59-K118834-0022-051-D01C-35St
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K118834-0022-051-D01C-35St
CCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATTCCGGA
CGGAGCGGGGTCGCTGCCGACCGGCTCATCCTCGATCCGGGGATGGGATTTTTCTTGAGC
CCCGCACCGGAAACATCGCTGCACGTGCTGTCGAACCTTCAAAAGCTGAAGTCGGCGTTG
GGGCTTCCGCTATTGGTCTCGGTGTCGCGGAAATCCTTCTTGGGGTTTCTTTTTTTTTTC
CCTTATATATAAAAATTTCAAACTAGGAAAAAAAAAAAAAACCGTTCGAAATTTCCTTGC
TTGTTTCCCTGAACAAGGGGGAGGCTTTCCCCAGGAATTCCCCTTTAAAGGGAAATTTCC
CAAAGGGTTTTTTAAATTGTACCCCGGGAAAAAAAATGCCACCCCTCCGAAAAAAATGGT
TTAAAAAAAAAAAAGATTTTCCTGATCCAAAAACCCTTTTTTTCTCAAACCCTTAAAACC
TTCCAAACTTTGGGGGGCGTTTTTAAAAAACAGGGATTGCTTCCTTTCGGGCCCCACCCA
CCCCCGGGTTTTTTTTCTTGTCCAAAAATTGGCCTCCCCCTTTTAAAAATTCCAAA

Last Updated on 06.12.2018 16:11