Sequences from confirmation amplicon


Confirmation sequences for line 051G10 and hit At4g23270 are shown.
Line ID 051G10
Locus At4g23270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12173241
Primers used for PCR 93qa - 8474, show primer details
   
Sequence Name 22-K115371-0022-051-G10C-93qa
GenBank Accession LN496244 [GenBank]
Sequencing primer gene specific primer, 93qa: 5'-AAATTTAGTACGTAGCCAAGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K115371-0022-051-G10C-93qa
ATTTGAATACGCTGTACAGACTCTACGATGAAAATGAAGCTAGACTGGACAAGAAGGTAC
AAGATCATAGGAGGAATTGCTAGAGGAATTCTTTATCTTCATCAAGATTCACGACTCACA
ATTATACATCGAGACCTCAAAGCAGGGAACATCCTCTTGGATGATGATATGAATCCTAAA
ATTGCAGATTTTGGAATGGCGAGAATATTTGGGATGGACCAAACAGAAGCCATGACAAGA
AGAGTAGTTGGAACTTAGTAAGTATTGCTCTTGCCACTTTTTTTTCATCAATAGACCGTA
ATACGTTTCAATCAACTAAAAAAAATAAGAATTATGGCAGTGGATCCATGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 02-K115370-0022-051-G10C-8409
GenBank Accession LN487162 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K115370-0022-051-G10C-8409
CATAATTCTTATTTTTTTTAGTTGATTGAAACGTATTACGGTCTATTGATGAAAAAAAAG
TGGCAAGAGCAATACTTACTAAGTTCCAACTACTCTTCTTGTCATGGCTTCTGTTTGGTC
CATCCCAAATATTCTCGCCATTCCAAAATCTGCAATTTTAGGATTCATATCATCATCCAA
GAGGATGTTCCCTGCTTTGAGGTCTCGATGTATAATTGTGAGTCGTGAATCTTGATGAAG
ATAAAGAATTCCTCTAGCAATTCCTCCTATGATCTTGTACCTTCTTGTCCAGTCTAGCTT
CATTTTCATCGTAGAGTCTGTACAGCGTATTTCAAATGTTAAGAACAAAGAATGGCATAC
AATTTGAAGATATCTTGGCTACTACTTAAATT

Last Updated on 06.12.2018 16:11