Sequences from confirmation amplicon


Confirmation sequences for line 052E04 and hit At3g07540 are shown.
Line ID 052E04
Locus At3g07540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2407208
Primers used for PCR 04fm - 3144, show primer details
   
Sequence Name 18-K111392-0022-052-E04C-04fm
GenBank Accession LN494362 [GenBank]
Sequencing primer gene specific primer, 04fm: 5'-TAGAGAACATCGGAGGTAGAAGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K111392-0022-052-E04C-04fm
TCTTGGAGGGGGACATGGAAGAGTTTGTTGTTGGGATTGGCTAATGTCTGAAGCTAAGCT
CTTGGATTCATCTTTGAAATGACTGGTTTGCCCTCGCCACCGTCCGTAGAAGAAAAAGGC
CAAGGCGATTAATGTTGCGGCGGCGAGCACGGCGGAGATAGCAGGAATAAGGAGTGTACG
GGAAGGAGTTTGCGGTTTAGGGGAGCGAGGGAGGACGAAACGACAATCTAAATGAAATGA
ACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTAC
GTCAGTGGAGATATCACATCAATCCA
   
Sequence Name 02-K111391-0022-052-E04C-3144
GenBank Accession LN487093 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K111391-0022-052-E04C-3144
CGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTAGATTGTCGTTTCGTCCTCCC
TCGCTCCCCTAAACCGCAAACTCCTTCCCGTACACTCCTTATTCCTGCTATCTCCGCCGT
GCTCGCCGCCGCAACATTAATCGCCTTGGCCTTTTTCTTCTACGGACGGTGGCGAGGGCA
AACCAGTCATTTCAAAGATGAATCCAAGAGCTTAGCTTCAGACATTAGCCAATCCCAACA
ACAAACTCTTCCATGTCCCCCTCCAAGAAACAACAACACTCAAAACAAACTCTCTGTAGC
TCCTTCTACCTACAATGTTCTCTAAA

Last Updated on 06.12.2018 16:11