Sequences from confirmation amplicon


Confirmation sequences for line 053B12 and hit At3g02040 are shown.
Line ID 053B12
Locus At3g02040 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:349662
Primers used for PCR 02fp - 8474, show primer details
   
Sequence Name 31-K111392-0022-053-B12C-02fp
GenBank Accession LN500217 [GenBank]
Sequencing primer gene specific primer, 02fp: 5'-ATCAATCTCACAGGAAGGAAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K111392-0022-053-B12C-02fp
GTCTCTAAAGCCATTCATGTCTCGGAAGTGCCAAGCCTCGACCACTTCCCTGAGAACCCG
TCGCTCATTTGCTCCTCTCGCAAAGCGAATAACAAGTTTGTGGTGGTTGGCCATAGAGGA
CACGGCATGAACATGTCGCAGTCGCCGGATCTAAGGTTCTCTGCTCTCAAGGAGAACTCC
ATCCTCTCCTTTAATGCCGCTTCAAAATTCCCTCTCGACTTTATCGAATTCGATGTTCAG
GTATTTACTTATTTCTTTCAATCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGC
   
Sequence Name 15-K111391-0022-053-B12C-8409
GenBank Accession LN493030 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K111391-0022-053-B12C-8409
TCGGATGNAGAATAGTAATACCTGAACATCGAATTCGATAAAGTCGAGAGGGAATTTTGA
AGCGGCATTAAAGGAGAGGATGGAGTTCTCCTTGAGAGCAGAGAACCTTAGATCCGGCGA
CTGCGACATGTTCATGCCGTGTCCTCTATGGCCAACCACCACAAACTTGTTATTCGCTTT
GCGAGAGGAGCAAATGAGCGACGGGTTCTCAGGGAAGTGGTCGAGGCTTGGCACTTCCGA
GACATGAATGGCTTTTAGAGACATATTTCTATTTTTAGAAAGAAAAAAGGGCTCGTCTTT
CTTTCTTTCTTTCCTTCCTGTNAGATTGA

Last Updated on 06.12.2018 16:11