Sequences from confirmation amplicon


Confirmation sequences for line 054C03 and hit At1g24807 are shown.
Line ID 054C03
Locus At1g24807 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8772646
Primers used for PCR PC30 - 8474, show primer details
   
Sequence Name 01-K103785-0022-054-C03C-PC30
GenBank Accession LN486544 [GenBank]
Sequencing primer gene specific primer, PC30: 5'-CATTTAAGCTGTGGATGCATAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K103785-0022-054-C03C-PC30
TGATACGGGAAATAAGATGTCTTGTTTATATGCAACTAACCTCCAAATGCTTCTCCTATA
CACTGCAAACCCATACATACTCCAAATAAAGGAACAAGTGGTCCGAGTTCCAAAACAGTT
TGCAAGGAAATCCCAGAGTCTTGAGGGGTACCTATCAAATTCAAGAAAAGTGTTATCCAC
TTCTCCAGCATATAATTATAGGAGAAATTTCAGAGGCCAAAATATTTACCAGGTCCAGGA
GAAATCAACACTCCTCTTGGATTTTTTCTGCAAGAGGGAGAGAGTAATGAGTATGATGGT
CAATATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGACCGCAG
CGTTATT
   
Sequence Name 17-K103786-0022-054-C03C-8409
GenBank Accession LN493821 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K103786-0022-054-C03C-8409
GCCTATTGACCTCAACTCATTACTCTCTCCCTCTTGCAGAAAAAATCCAAGAGGAGTGTT
GATTTCTCCTGGACCTGGTAAATATTTTGGCCTCTGAAATTTCTCCTATAATTATATGCT
GGAGAAGTGGATAACACTTTTCTTGAATTTGATAGGTACCCCTCAAGACTCTGGGATTTC
CTTGCAAACTGTTTTGGAACTCGGACCACTTGTTCCTTTATTTGGAGTATGTATGGGTTT
GCAGTGTATAGGAGAAGCATTTGGAGGTTAGTTGCATATAAACAAGACATCTTATTTTCC
CGTATCAGTGTTCAAACATGGTTTTGTTCTTTATTCTATGCATCCACAGCTTAAATG
Primers used for PCR 48oc - 8474, show primer details
   
Sequence Name 41-K114678-0022-054-C03C-48oc
GenBank Accession LN504177 [GenBank]
Sequencing primer gene specific primer, 48oc: 5'-TCGAGCTCATCACTAGGAAATGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K114678-0022-054-C03C-48oc
GAACCACCAGATTTGTTTTATTACACATTTTTACAGACTTATGATTAACTATATTAGACA
ACACACAAAAAAAATTGATTTTGATTCATACTTTGATAATCCAGAGAACAAGCCTTCTTC
TCCTTTCTCATCATAGTGAACCATTGAGCTTTTCCCATGCATAACACCAAATGGTGACCG
CACAATCTTTCCTAATCAATGTTCAAACAAAATTGCATTTAAGCTGTGGATGCATAGAAT
AAAGAACAAAACCATGTTTGAACACTGATACGGGAAAATAAGATGTCTTGTTTATATGCA
ACTAACCTCCAAATGCTTCTCCTATACACTGCAAACCCATACATACTCCAAATAAAGGAA
CAAGTGGTCCGAGTTCCAAAACAGTTTGCAAGGAAATCCCAGAGTCTTGAGGGGTACCTA
TCAAATTCAAGAAAAGTGTTATCCACTTCTCCAGCATATAATTATAGGAGAAATTTCAGA
GGCCAAAATATTTACCAGGTCCAGGAGAAATCAACACTCCTCTTGGATTTTTTCTGCAAG
AGGGAGAGAGTAATGAGTATGATGGTCAATATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCC
   
Sequence Name 17-K114677-0022-054-C03C-8409
GenBank Accession LN493960 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114677-0022-054-C03C-8409
CATACTCATTACTCTCTCCCTCTTGCAGAAAAAATCCAAGAGGAGTGTTGATTTCTCCTG
GACCTGGTAAATATTTTGGCCTCTGAAATTTCTCCTATAATTATATGCTGGAGAAGTGGA
TAACACTTTTCTTGAATTTGATAGGTACCCCTCAAGACTCTGGGATTTCCTTGCAAACTG
TTTTGGAACTCGGACCACTTGTTCCTTTATTTGGAGTATGTATGGGTTTGCAGTGTATAG
GAGAAGCATTTGGAGGTTAGTTGCATATAAACAAGACATCTTATTTTCCCGTATCAGTGT
TCAAACATGGTTTTGTTCTTTATTCTATGCATCCACAGCTTAAATGCAATTTTGTTTGAA
CATTGATTAGGAAAGATTGTGCGGTCACCATTTGGTGTTATGCATGGGAAAAGCTCAATG
GTTCACTATGATGAGAAAGGAGAAGAAGGCTTGTTCTCTGGATTATCAAAGTATGAATCA
AAATCAATTTTTTTTGTGTGTTGTCTAATATAGTTAATCATAAGTCTGTAAAAATGTGTA
ATAAAACAAATCTGGTTGGTTTCAGCCCTTTCATTGTAGGTAGATATCACAGTCTCGTGA
TCGAAAAAGATACATTTCCTAGTGAGAAGCTCGA

Last Updated on 06.12.2018 16:11