Sequences from confirmation amplicon


Confirmation sequences for line 054E10 and hit At5g56560 are shown.
Line ID 054E10
Locus At5g56560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22901553
Primers used for PCR 04vg - 3144, show primer details
   
Sequence Name 34-K117737-0022-054-E10C-04vg
GenBank Accession LN501578 [GenBank]
Sequencing primer gene specific primer, 04vg: 5'-AAGTCAGGGAAAAGGAAGAAAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K117737-0022-054-E10C-04vg
GCCGACATGTCTTTGAAAATCTGTCCATGGACTTTAACTTCTTTAACATGCGATGATTTC
TGTTTCTGCATCTTGTCGAGAACGTCGCCATCTTTAGACATGTAGCGTTTGCTAGAATGT
AGCCTGCCACATTCCTCTCTTGCTCTGTGCCTTCATATTGTCTCCATTCAAAGATCTCGA
GATGCTCCGATAAACATTTAGCAACAACTGCTGGTTCTTCCCAGAGGGTCATCGGATCAT
TGTAATTGAATTTATGTTTCTGAAACGAAATAATGAAACGATCACTAGTTTAAACAGGCA
CTATGTTCATCGGATCATTGTAACGTGCACTAGTTCTTAAACATCGTTCGTTCAAAAAGA
AAAGCAGAAACAAAAAGATGATAGAATGAAATAGTGATTCTACAAATCTTTTTTATCATC
TTAGATCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTA
CCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATA
GTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCAC
   
Sequence Name 02-K117736-0022-054-E10C-3144
GenBank Accession LN487216 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K117736-0022-054-E10C-3144
TATACGGAAGTTCATTTCGTTTGGAGAGGACTAGGTACCCGGGGATCAGATTGTCGTTTC
CCGCCTTCGGATTTAACTATCAGTGTTTGATCTAAGATGATAAAAAAGATTTGTAGAATC
ACTATTTCATTCTATCATCTTTTTGTTTCTGCTTTTCTTTTTGAACGAACGATGTTTAAG
AACTAGTGCACGTTACAATGATCCGATGAACATAGTGCCTGTTTAAACTAGTGATCGTTT
CATTATTTCGTTTCAGAAACATAAATTCAATTACAATGATCCGATGACCCTCTGGGAAGA
ACCAGCAGTTGTTGCTAAATGTTTATCGGAGCATCTCGAGATCTTTGAATGGAGACAATA
TGAAGGCACAGAGCAAGAGAGGAATGTGGCAGGCTACATTCTAGCAAACGCTACATGTCT
AAAGATGGCGACGTTCTCGACAAGATGCAGAAACAGAAATCATCGCATGTTAAAGAAGTT
AAAGTCCATGGACAGAGTTTCAAAGGCATGTCGGCTTGTGTTTGACTAAACTCTGATGTG
TGTTGTGTGTTTCTACAAGACACAAGATGTTTTCTTAGTTATCTTTCTTCCTTTTCCCTG
AC

Last Updated on 06.12.2018 16:11