Sequences from confirmation amplicon


Confirmation sequences for line 055C08 and hit At5g58860 are shown.
Line ID 055C08
Locus At5g58860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23766855
Primers used for PCR MR21 - 3144, show primer details
   
Sequence Name 01-K015284-022-055-C08C-3144
GenBank Accession LN486362 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K015284-022-055-C08C-3144
AGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCA
TTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCATTTGGAGCCTATCTGCCT
CGTCGAACTCTAACGGCTCCTCCGTCCATTTCTCCTGATCATTGCCACGTGTCTCCTTCA
GAACCATCGACAACTCGTTAACGATCTTCGTTTCCACCTCCCGGTTATTCATGACGAGCC
AGAAGAACCAGCTCAAGGCCACCGAAGAAGTGTCACGGCCCGCGAGAACAAAGTTAAGCG
CGATACGCTGAAGAACATCTGTTGGAAGAACGTTACCGTTAACGTCACGTTTCTTCAAGA
AACGTGACAAAAGATCAT
   
Sequence Name 02-K015284-022-055-C08C-MR21
GenBank Accession LN486812 [GenBank]
Sequencing primer gene specific primer, MR21: 5'-GATGATCTTTTGTCACGTTTCTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K015284-022-055-C08C-MR21
GTTAACGGTAACGTTCTTCCAACAGATGTTCTTCAGCGTATCGCGCTTAACTTTGTTCTC
GCGGGCCGTGACACTTCTTCGGTGGCCTTGAGCTGGTTCTTCTGGCTCGTCATGAATAAC
CGGGAGGTGGAAACGAAGATCGTTAACGAGTTGTCGATGGTTCTGAAGGAGACACGTGGC
AATGATCAGGAGAAATGGACGGAGGAGCCGTTAGAGTTCGACGAGGCAGATAGGCTCCAA
ATGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAG
AGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATAT
CACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCC
TCGTGGGTGGGGGTCCATCTTTGGGACCACTGTCGGCAGAGGCATCTTCAACGATGGCCT
TTCCTTTATCGCAATGATGGCATTTGTAGGAGCCACCTTCCTTTTCCACTATCTTCACAA
TAAAGTGACAGATAGCTGGGCAATGGAATCCGAGGAGGTTTCCGGATATTACCCTTTGTT
GAAAAGTCTCAATTGCCCTTTGGT
Primers used for PCR FI23 - 8474, show primer details
   
Sequence Name 12-K028095-022-055-C08C-8409
GenBank Accession LN491427 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K028095-022-055-C08C-8409
GATAAATTAAAATTCTTTTATTTTTTCTCTCTTTAATCATTCGCATTCTTTTTTTGTCGA
CGGCTAACTATTTGCATTCATGGAAACATTAACTATAACGCTTTTGGCTAGGCGAGAATT
ACATAGAAAACGTAAAAATTCAGAAATAATTAGATATTGCACAAAGATTGATTTATGTCT
AAACATGTATATCAACTTTTATAGAAACACCTGAAATAAATCTTTTATGACTAGTTGACG
AATTCCATTGTTTTTTCTTTTAAGCTGAATATTACAGATTTCTTTTTCTGCTAGCTCTAG
TATCAAATGACTGTTACAAACTTACAATCATAATTTGAGTATTTAATTTGTGTTATTTTT
ACCAAAATAAAAAAGGGAAGAATTGACGCAAAATTACTCATCATGTATTCATAATAAAGA
GCTGTGTGTAACGTATAGTAACGTTTCAAATGACCA
   
Sequence Name 12-K028095-022-055-C08C-FI23
GenBank Accession LN491428 [GenBank]
Sequencing primer gene specific primer, FI23: 5'-GACGTATTTGAAATCCTGGTCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K028095-022-055-C08C-FI23
CAGCTCTTTATATGAAACATGATGAGTAATTTTGCGTCAATTCTTCCCTTTTTTATTTTG
GTAAAAATAACACAAATTAAATACTCAAATTATGATTGTAAGTTTGTAACAGTCATTTGA
TACTAGAGCTAGCAGAAAAAGAAATCTGTAATATTCAGCTTAAAAGAAAAAACAATGGAA
TTCGTCAACTAGTCATAAAAGATTTATTTCAGGTGTTTCTATAAAAGTTGATATACATGT
TTAGACATAAATCAATCTTTGTGCAATATCTAATTATTTCTGAATTTTTACGTTTTCTAT
GTAATTCTCGCCTAGCCAAAAGCGTTATAGTTAATGTTTCCATGAATGCAAATAGTTAGC
CGTCAACAAAAAAAGAATGCAAATAATTAAAGAGAGAAAAATAAAAGATTTTAATTTATC
ACCTGAGGCACAGAAGGGTATAAACGCAGCGTTTCAGCCAAAGGAGCCTTGAGGTAAGCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCT
ACATT

Last Updated on 06.12.2018 16:11