Sequences from confirmation amplicon


Confirmation sequences for line 055G07 and hit At3g13160 are shown.
Line ID 055G07
Locus At3g13160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4231278
Primers used for PCR KA17 - 8474, show primer details
   
Sequence Name 27-K105138-0022-055-G07C-KA17
GenBank Accession LN498381 [GenBank]
Sequencing primer gene specific primer, KA17: 5'-CCTTGTTCTCAATCTCATCAATCA-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K105138-0022-055-G07C-KA17
TCCCTTGATCAAAGTATTGTAAGATGCAACATCTGGTTCAATCGAGAGCTTACCTGGCAA
CTCCTTGAAGATACCTTCAACCAAATCAAACTTCTTGGAGTTCACACAGGCATTCAGTAA
TGCGTTGAAAGACAACGCTGTTCTCTTGCAGTTTCTCTCAGGCATTTCGTCGAACACTTT
CTGTGCATTCTCAAACATACCGACTCGTCCATAGAGATTGATAATCCTCGCCACGAATCC
TTCCTTTGACATGTTTGGATACTTGTTCTGCTCCTCCAGGATCTCCTCGACCCACTCAAA
CTTCTTGGCTGCGGCGAGACGGCGAACGGTTCTCTCATAGACAGCTATATTCTTCCGGAA
CCACTCTGCTTGGCAGGCTTTCTTGAACTTCTCCGTGATGAATTTGGGGTCGCGTTCGTC
ATTTACGAGGGTGATGAGGGACGGTTTGGGTGGCGAGGGTGTGGCTGCAGCAGCGGTAAC
GGCGGAGAAGAAGCGGCGATTCGTGTGGGTGCTGAAGCTGAAGTTGCCACGAAGGAGAAA
GCGAGAGAGAGACGACATCGTTTTTAGCTGTATGCAAAGGTTTTTCTCTTCTTTTTTTTG
GATCGGAGTGTTCAAAGGTTTTTAGTCAGGGGAACGGCTTTAATACTTCAAATTAGGTAA
ATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTA
   
Sequence Name 11-K105139-0022-055-G07C-8409
GenBank Accession LN491106 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K105139-0022-055-G07C-8409
AATTGAATATATCCTGATTTACCTAATTTGAAGTATTAAAGCCGTTCCCCTGACTAAAAA
CCTTTGAACACTCCGATCCAAAAAAAAGAAGAGAAAAACCTTTGCATACAGCTAAAAACG
ATGTCGTCTCTCTCTCGCTTTCTCCTTCGTGGCAACTTCAGCTTCAGCACCCACACGAAT
CGCCGCTTCTTCTCCGCCGTTACCGCTGCTGCAGCCACACCCTCGCCACCCAAACCGTCC
CTCATCACCCTCGTAAATGACGAACGCGACCCCAAATTCATCACGGAGAAGTTCAAGAAA
GCCTGCCAAGCAGAGTGGTTCCGGAAGAATATAGCTGTCTATGAGAGAACCGTTCGCCGT
CTCGCCGCAGCCAAGAAGTTTGAGTGGGTCGAGGAGATCCTGGAGGAGCAGAACAAGTAT
CCAAACATGTCAAAGGAAGGATTCGTGGCGAGGATTATCAATCTCTATGGACGAGTCGGT
ATGTTTGAGAATGCACAGAAAGTGTTCGACGAAATGCCTGAGAGAAACTGCAAGAGAACA
GCGTTGTCTTTCAACGCATTACTGAATGCCTGTGTGAACTCCAAGAAGTTTGATTTGGTT
GAAGGTATCTTCAAGGAGTTGCCAGGTAAGCTCTCGATTGAACCAGATGTTGCATCTTAC
AATACTTTGATCAAGGGATTGTGTGGAAAGGGTTCCTTCACTGAAGCTGTTGCGTTGATT
GATGA

Last Updated on 06.12.2018 16:11