Sequences from confirmation amplicon


Confirmation sequences for line 056A05 and hit At4g39950 are shown.
Line ID 056A05
Locus At4g39950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18526507
Primers used for PCR IF33 - 8474, show primer details
   
Sequence Name 49-K101385-0022-056-A05C-8409
GenBank Accession LN506776 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K101385-0022-056-A05C-8409
TTTCTGTAGATTTCTACAACATGGGCTACTTTTTTGTTTGTCTCTTTTGATTACTTTGGT
CAAAGACAGATGCATGCAACTTAATTGTATTTATTTTTATGTTATACAAAAATTAAAGAT
CCAAAATTAATAAAAGCTGGTATATATGTTTATAATGAATAGGAGCTTGTAATGGCGGCG
CCAGACAATCCATCAAACGCCGTGGAATGGGCCATGGCGGAGATGGTGAACAAACCGGAG
ATTCTCCGTAAAGCAATGGAAGAGATCGACAGAGTCGTCGGGAAAGAGAGACTCGTTCAA
GAATCCGACATCCCAAAACTAAACTACGTCAAAGCTATCCTCCGCGAAGCTTTCCGTCTC
CATCCCGTCGCCGCCTTCAACCTCCCCCACGTGGCACTTTCTGACACAACCGTCGCCGGA
TATCACATCCCTNANGGAAGTCAAGTCCTTCTTAGCCCATA
   
Sequence Name 49-K101391-0022-056-A05C-IF33
GenBank Accession LN506777 [GenBank]
Sequencing primer gene specific primer, IF33: 5'-CATATCGGCTAAGAAGGACTTGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K101391-0022-056-A05C-IF33
GGCGACGGTGTGTCAGAAGTGCCACGTGGGGGAGGTTGAAGGCGGCGACGGGATGGAGAC
GGAAAGCTTCGCGGAGGATAGCTTTGACGTAGTTTAGTTTTGGGATGTCGGATTCTTGAA
CGAGTCTCTCTTTCCCGACGACTCTGTCGATCTCTTCCATTGCTTTACGGAGAATCTCCG
GTTTGTTCACCATCTCCGCCATGGCCCATTCCACGGCGTTTGATGGATTGTCTGGCGCCG
CCATTACAAGCTCCTATTCATTATAAACATATATACCAGCTTTTATTAATTTTGGATCTT
TAATTTTTGTATAACATAAAAATAAATACAATTAAGTTGCATGCATCTGTCTTTGACCAA
AGTAATCAAAAGAGACAAACAAAAAAGTAGCCCATGTTTGTAGAAATCTACATGAAAATG
GTAAAATAAAACAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATNTCCGC

Last Updated on 06.12.2018 16:11