Sequences from confirmation amplicon


Confirmation sequences for line 056C07 and hit At1g56130 are shown.
Line ID 056C07
Locus At1g56130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20996016
Primers used for PCR 19xh - 8474, show primer details
   
Sequence Name 81-K119123-0022-056-C07C-19xh
GenBank Accession LN514124 [GenBank]
Sequencing primer gene specific primer, 19xh: 5'-CACAACACCAAAGGCATACACATC-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K119123-0022-056-C07C-19xh
CGCAGATACCCACTGCATTGATACAGCAACATCACTAACATGTCTCACTAATTTGTAATT
GTGATGCAAACCAAAGGTCAGAGTTTGTGGATTACTTACATCGTCCCTGCCACTCGGGTA
CTTATGTGGGTTTTCTTGTCATCGTACAGTTTTGCAAGTCCAAAATCAGAAATTTGTGGG
ACCAGTCTAGAGTCGAGCAAAATGTTACTGGCCTTCACATCCCTGTGTACTATGCGAACA
CTCGCCTCCTCGTGGAGATAAACTAGACCTCTGGCTACTCCCAGGCATATCTCATAACGG
GTTGACCAATCAAGATGTAAGGTCTTATCCCCTGAAACATGCAAACCAAGGGCATAAATG
AGTACTTAAGGTTCGCAGAAGCAGTGATAGATAAGTCATGTTTCTGTTCATTACCAAATA
GCGCCTGATCAAGACTTCCATTAGGTAGATACTCATATACGAGCATACGATGCTCTCCTT
CAAACCTCAATGGCTTTCCCTATACAGGATAAACCTCAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 58-K119111-0022-056-C07C-8409
GenBank Accession LN509844 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K119111-0022-056-C07C-8409
TTACATTGAATATATCCTGAGGTTTATCCTGTATAGGGAAAGCCATTGAGGTTTGAAGGA
GAGCATCGTATGCTCGTATATGAGTATCTACCTAATGGAAGTCTTGATCAGGCGCTATTT
GGTAATGAACAGAAACATGACTTATCTATCACTGCTTCTGCGAACCTTAAGTACTCATTT
ATGCCCTTGGTTTGCATGTTTCAGGGGATAAGACCTTACATCTTGATTGGTCAACCCGTT
ATGAGATATGCCTGGGAGTAGCCAGAGGTCTAGTTTATCTCCACGAGGAGGCGAGTGTTC
GCATAGTACACAGGGATGTGAAGGCCAGTAACATTTTGCTCGACTCTAGACTGGTCCCAC
AAATTTCTGATTTTGGACTTGCAAAACTGTACGATGACAAGAAAACCCACATAAGTACCC
GAGTGGCAGGGACGATGTAAGTAATCCACAAACTCTGACCTTTGGTTTGCATCACAATTA
CAAATTAGTGAGACATGTTAGTGATGTTGCTGTATCAATGCAGTGGGTATCTTGCGCCAG
AGTATGCCATGCGTGGACATCTAACAGAGAAAACGGATGTGTATGCCTTGGGTGTTGTG

Last Updated on 06.12.2018 16:11