Sequences from confirmation amplicon


Confirmation sequences for line 056E04 and hit At1g10385 are shown.
Line ID 056E04
Locus At1g10385 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3405421
Primers used for PCR 05sm - 8474, show primer details
   
Sequence Name 42-K116549-0022-056-E04C-05sm
GenBank Accession LN504602 [GenBank]
Sequencing primer gene specific primer, 05sm: 5'-GGTGTTTTGCAAGTGTTTAACTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K116549-0022-056-E04C-05sm
AACGAAGAGAATCCAGTTCATAGAATAGTTAAAGTAGCTGAAACTGAGTCCCAACAAACG
GCTTTGTTAGTCAATGCGCTTCTTTTAGCAGACGAGCTAATTCCACGTTCTGCTTCAAGG
ATTTTGCCACAAGGTACGAGCCAATCCACTCCAAGAAGAGGTTCCTCAGATAGGCAGAAC
CGACCAGAGCAAAGAGAATGGAAGAAGAAGCTTCAAAGATCAGTGGATCGACTAAGAGAT
AGCTTCTGCAGACAACATGCACTTGAACTCATCTTCACTGAAGAAGGAGAAGTCCGTCTC
AGCTCTGAGATTTATATCCTCATGGACGAAACCACTGAAGAGCCTGAATGGTTCCCTTCA
CCAATTTTCCAGGAACTGTTTGCGAAATTGACGAGGATAGCCATGATTGTGAGCGATATG
TTTGTGGGGAGAGAGAGATTCGCGACGATATTGCTGATGAGACTGACGGAGACAGTGATT
CTTTGGATATCTTTGTCCGGTTATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATA
   
Sequence Name 02-K116548-0022-056-E04C-8409
GenBank Accession LN487194 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K116548-0022-056-E04C-8409
TATATCCTGGATACCGGACAAAGATATCCAAAGAATCACTGTCTCCGTCAGTCTCATCAG
CAATATCGTCGCGAATCTCTCTCTCCCCACAAACATATCGCTCACAATCATGGCTATCCT
CGTCAATTTCGCAAACAGTTCCTGGAAAATTGGTGAAGGGAACCATTCAGGCTCTTCAGT
GGTTTCGTCCATGAGGATATAAATCTCAGAGCTGAGACGGACTTCTCCTTCTTCAGTGAA
GATGAGTTCAAGTGCATGTTGTCTGCAGAAGCTATCTCTTAGTCGATCCACTGATCTTTG
AAGCTTCTTCTTCCATTCTCTTTGCTCTGGTCGGTTCTGCCTATCTGAGGAACCTCTTCT
TGGAGTGGATTGGCTCGTACCTTGTGGCAAAATCCTTGAAGCAGAACGTGGAATTAGCTC
GTCTGCTAAAAGAAGCGCATTGACTAACAAAGCCGTTTGTTGGGACTCAGTTTCAGCTAC
TTTAACTATTCTATGAACTGGATTCTCTTCGTTTTCAGCTGAACCGGGCAATGCGTTGAT
CAAGAGATCAACGTATGAGTTAA

Last Updated on 06.12.2018 16:11